Expression of Genes Encoding Membrane-bound Hydrogenase in Pseudomonas hydrogenovora under Autotrophic Condition Is Dependent on Two Different Promoters

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概要

• 論文の詳細を見る

• Expression of a membrane-bound hydrogenase of the hydrogen-utilizing bacterium Pseudomonas hydrogenovora was induced specifically in autotrophic condition. Dot blot and primer extension analysis showed that the transcription of the hydrogenase gene started from the region upstream of a hydrogenase structural gene, hupS, which contained three putative σ^<54>-type promoter sequences (P_<hup1>, P_<hup2>, and P_<hup3>). The lacZ-fusion analysis of the hupS-upstream region combined with site-directed mutagenesis showed that P_<hup1> and P_<hup3> would be essential for a transcriptional initiation. It was also found that the region upstream of P_<hup> sequences was concerned with regulation of transcription.

• 社団法人日本農芸化学会の論文
• 1997-12-23

著者

• Ohtsuki Takashi

  Department Of Applied Chemistry And Biotechnology Faculty Of Engineering University Of Yamanashi

• OKAZAKI MITSUO

  Department of Applied Biology, Shinshu University

• Ohtsuki Takashi

  Department Of Applied Biology Faculty Of Textile Science And Technology Shinsyu University

• SHIMOSAKA Makoto

  Gene Research Center, Shinsyu University

• Shimosaka Makoto

  Gene Research Center Shinsyu University

• Okazaki Mitsuo

  Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University

• Okazaki Mitsuo

  Department Of Applied Biology Faculty Of Textile Science And Technology Shinsyu University:gene Rese

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