Effects of Kinetin on Formation of Scopoletin and Scopolin in Tobacco Tissue Cultures
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概要
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Kinetin increased the formation of scopoletin and scopolin in tobacco tissue cultures. The formation of scopolin was increased more in the presence of both kinetin (KIN) and L-phenylalanine (Phe) in the medium than in the case of either KIN or Phe alone. The activity of L-phenylalanine ammonia-lyase (PAL) was increased by the addition of KIN. The increase of PAL activity was inhibited by actinomycin-D and cycloheximide added at the same time as the KIN, but actinomycin-D did not inhibit the increase when added at 8 hr after the addition of KIN at which time cycloheximide inhibited it still. The incorporation of [U-14C]-Phe into scopoletin and scopolin was stimulated by KIN, but its incorporation into protein and lignin was not affected. These results suggest that KIN increased de novo synthesis of PAL through its mRNA, and the enhanced activity of PAL resulted in the increase of scopoletin and scopolin formation.
- 社団法人 日本農芸化学会の論文
著者
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Miura Yoshiharu
Department Of Biochemical Engineering Faculty Of Pharmaceutical Sciences Osaka University
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Okazaki Mitsuo
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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HINO Fumitsugu
Department of Biochemical Engineering, Faculty of Pharmaceutical Sciences, Osaka University
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