Isolation and Transcriptional Analysis of a Cellulase Gene(cel1) from the Basidiomycete Irpex lacteus

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概要

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• A gene(named cel1) homologous to the cellobiohydrolase I gene(cbh1) of Trichoderma reesei was isolated and sequenced from the white rot basidiomycete Irpex lacteus MC-2. The cel1 open reading frame consists of 1551 bp, which is interrupted by two introns, encoding a polypeptide of 517 amino acid residues with a calculated molecular mass of 54, 522 Da. The deduced amino acid sequence showed that CEL1(the protein encoded by cel1) has a modular structure consisting of a catalytic domain of 449 amino acids and a C-terminal cellulose-binding domain(CBD) of 36 amino acids separated by a proline-, serine-, threonine-rich linker region of 32 amino acids. The CEL1 catalytic domain is homologous with fungal cellobiohydrolases(CBHs) belonging to family 7 of the glycosyl hydrolases. The transcription of cel1 was induced in the presence of various cellulosic substrates and repressed by glucose. It was therefore concluded that the reported sequence represents the first cellulase gene isolated from the basidiomycete Irpex.

• 社団法人日本生物工学会の論文
• 1999-01-25

著者

• Hamada N

  Laboratory Of Applied Microbiology Graduate School Of Marine Science And Technology Tokyo University

• OKAZAKI MITSUO

  Department of Applied Biology, Shinshu University

• SHIMOSAKA MAKOTO

  Department of Applied Biology, Shinshu University

• Shiroishi Masahiro

  Department Of Physics Graduate School Science University Of Tokyo

• KANDA Takahisa

  Department of Chemistry and Material Engineering, Faculty of Engineering, Shinshu University

• Kanda Takahisa

  Department Of Chemistry And Material Engineering Faculty Of Engineering Shinshu University

• Shimosaka M

  信州大・繊維・応生系

• Shimosaka Makoto

  Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University

• KODAIRA Ritsuko

  Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University

• HAMADA NAOKO

  Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University

• FUSE NAOKI

  Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University

• OKUMURA RYO

  Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University

• Okumura Ryo

  Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University

• Okazaki M

  Dep. Of Applied Biology Fac. Of Textile Sci. And Technol. Shinshu Univ.

• Okazaki Mitsuo

  Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University

• 城石 正弘

  Division Of Applied Biology Faculty Of Textile Science And Technology Shinshu University

• Fuse Naoki

  Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University

• Kodaira Ritsuko

  Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University

• Kuroyanagi Tomoko

  Faculty Of Textile Science And Technology Shinshu University

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