Mode of Action of Cellulases on Dyed Cotton with a Reactive Dye

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概要

• 論文の詳細を見る

• Cotton woven fabrics which were previously dyed with a reactive dye were treated with a commercial cellulase preparation. Dyeing with a reactive dye for cotton apparently inhibited the weight loss activity and saccharification activity of cellulase. In addition, dyed cotton was treated with highly purified cellulases which were exo-type cellulases (Cellobiohydrolase I (CBH I) and Cellobiohydrolase II (CBH II)) and endo-type cellulase (Endoglucanase II (EG II)). Exo-type cellulases were inhibited more than endo-type cellulase by dyeing in the case of saccharification activity. CBH I was severely inhibited by dyeing as compared with CBH II or EG II from the viewpoint of morphological changes in the fiber surface. Dyes on the cellulose substrates severely influenced CBH I in spite of the rare modification, because CBH I hydrolyzed cellulose with true-processive action. The change in the activity of each cellulase component on dyed cotton can affect the synergistic action of cellulases.

• 社団法人 日本農芸化学会の論文
• 2005-01-23

著者

• NOZAKI Kouichi

  Department of Applied Bioscience, Graduate School of Agriculture, Hokkaido University

• Nozaki Kouichi

  Department Of Chemistry And Material Engineering Faculty Of Engineering Shinshu University

• KANDA Takahisa

  Department of Chemistry and Material Engineering, Faculty of Engineering, Shinshu University

• AMANO Yoshihiko

  Department of Chemistry and Material Engineering, Faculty of Engineering, Shinshu University

• Kanda Takahisa

  Department Of Chemistry And Material Engineering Faculty Of Engineering Shinshu University

• Amano Yoshihiko

  Department Of Chemistry And Material Engineering Faculty Of Engineering Shinshu University

• YAMADA Minoru

  Techno Center, Tokai Senko K. K.

• HORIKAWA Eisuke

  Department of Chemistry and Material Engineering, Faculty of Engineering, Shinshu University

• Nishizawa Kenichi

  Department Of Chemistry And Material Engineering Faculty Of Engineering Shinshu University

• Arikawa Y

  Department Of Chemistry And Material Engineering Faculty Of Engineering Shinshu University

• Horikawa Eisuke

  Department Of Chemistry And Material Engineering Faculty Of Engineering Shinshu University

• Yamada Minoru

  Techno Center Tokai Senko K. K.

• Kuroyanagi Tomoko

  Faculty Of Textile Science And Technology Shinshu University

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