Action of Exo- and Endo-Type Cellulases from Irpex lacteus on Valonia Cellulose

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概要

• 論文の詳細を見る

• Cellulolytic mode of action of the two highly purified exo- and endo-type cellulases from Irpex lacteus on pure Valonia cellulose was investigated. Electron microscopy substantiated that both cellulases are adsorbed preferentially into the internal parts of microfibrils in the network structure of the cellulose at initial stages before enzymatic hydrolysis, and that the adsorption ratio of both cellulases onto the external surfaces of microfibrils increased with incubation time although this tendency was less remarkable with the exo-type cellulase than with the endo-type one. The exo-type cellulase exhibited relatively high activity producing cellobiose throughout 12-h incubation, while the endo-type cellulase produced small amounts of cellooligosaccharides. The degree of polymerization was far more suppressed by the endo-type cellulase than by the exo-type one. Degradation by the cellulases in typical exo- and endo-fashions yielded quite different morphological patterns in the microfibrils. Exo-type cellulase loosened the network structure of microfibrils and made them slightly thinner, while endo-type cellulase caused conspicuous swelling and dissolution of individual microfibrils.

• 社団法人日本生物工学会の論文
• 1994-05-25

著者

• Takai Mitsuo

  Department Of Applied Chemistry Faculty Of Engineering Hokkaido University

• OKAZAKI MITSUO

  Department of Applied Biology, Shinshu University

• Nomura Masafumi

  Wakayama Research Laboratories Kao Corporation

• Hoshino Eiichi

  Aset Euv Laboratory C-o Ntt Atsugi Research Center

• Hoshino E

  Tokyo Research Laboratories Kao Corporation

• Nozaki Kouichi

  Department Of Chemistry And Material Engineering Faculty Of Engineering Shinshu University

• KANDA Takahisa

  Department of Chemistry and Material Engineering, Faculty of Engineering, Shinshu University

• Kanda Takahisa

  Department Of Chemistry And Material Engineering Faculty Of Engineering Shinshu University

• Nomura M

  Department Of Chemistry Faculty Of Sciences Science University Of Tokyo

• HOSHINO EIICHI

  Household Products Research Laboratories, Kao Corporation

• NOMURA MASAFUMI

  Household Products Research Laboratories, Kao Corporation

• NISIZAWA KAZUTOSI

  Department of Fisheries, College of Agriculture and Veterinary Medicine, Nihon University

• Okazaki Mitsuo

  Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University

• Nishizawa Kenichi

  Department Of Chemistry And Material Engineering Faculty Of Engineering Shinshu University

• Nisizawa Kazutosi

  Department Of Botany Faculty Of Science Tokyo Kyoiku University

• Kuroyanagi Tomoko

  Faculty Of Textile Science And Technology Shinshu University

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