Kinetic Studies on Enzyme Production by Mictobes : (III) Process Kinetics of Glucamylase Production by Aspergillus niger

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The process kinetics of glucamylase production were studied with two adenine-requiring strains of Aspergillus niger originated from the strain U-20-2-5.The time courses of μ(specific growth rate) and ε(enzyme pruducting activity)in growing phase were in conformity with the kinetic model expressed by Eq. (17')or (18'), as expected from the physiological studies in which indirect evidences were presented concerning the remarkable stability of the specific mRNA; and the strong catabolite (or metebolite) repression of glucamylase formation.After cessation of growth, the specific mRNA seemed to be formed through the turmover utilization of decaying RNA. This was presumed from the fact that the incorporation of ^14C-guanine into RNA and the induced activity of glucamylase run parallel to one another in nongrowing maltose-cultures started with uninduced mycelia. These time courses could be adequately expressed by Eq. (23) and (23'), respectively, in which the rate of mRNA syntheses was assumed to be limited by the monomolecular rate of RNA decay. From the above was obtained the expression fitted in with the time course in the later phase of culture(Eq. (24) of (24')).The addition of guanine to nongrowing enzyme-producing cultures improved the rate of turnover utilization to from the specific mRNA without affecting the decayconstants, κ(for the mRNA) and λ(for cellular RNA).The temperature dependence of these decay constants was investigated with nongrowing cultures to obtain Arrhenius plots, which were straigh lines having the slopes corresponding to 9.2 and 18 kcal/mole for κ- and λ-steps, respectively.The activation energy for the activity of glucamylase formation was estimated at 20 kcal/mole.
公益社団法人日本生物工学会の論文
1967-12-25

Kinetic Studies on Enzyme Production by Mictobes : (III) Process Kinetics of Glucamylase Production by Aspergillus niger

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