Isolation and Characterization of Zygosaccharomyces rouxii Mutants Defective in Proton Pumpout Activity and Salt Tolerance
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概要
- 論文の詳細を見る
Two mutants defective in salt tolerance were identified among hygromycin B (HygB)-resistant mutants of Zygosaccharomyces rouxii. These mutants showed different phenotypes in terms of sensitibity towards high concentrations of glucose and KCl. Recovery of salt tolerance by the addition of KCl and CaCl_2 or by lowering pH (pH4.0) was different for the two mutants. Moreover, both mutants showed lowered plasma membrane (PM-) ATPase activity and proton pumpout activity. They exhibited neither growth nor proton pumpout ac-tivity in a medium containing 5% NaCl. The proton pumpout activity was inhibited by vanadate, an inhibitor or PM-ATPase, only when cells were incubated in the presence of more than 1% NaCl. Damage of the proton pumpout activity seems to be the reason for the salt sensitivity of both mutants. We showed that it was essential for Z. rouxii cells to pump out protons under a high salt environment using mutants defective in this ability.
- 社団法人日本生物工学会の論文
- 1995-01-25
著者
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OKAZAKI MITSUO
Department of Applied Biology, Shinshu University
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SHIMOSAKA MAKOTO
Department of Applied Biology, Shinshu University
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Hashimoto H
Department Of Applied Biology Faculty Of Textile Science & Technology Shinshu University
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YOSHIKAWA SHIGETOSHI
Food Technology Research Insititute of Nagano Prefecture
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MITSUI NOBUO
Department of Applied Biology, Faculty of Textile Science & Technology, Shinshu University
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CHIKARA KEN-ICHI
Department of Applied Biology, Faculty of Textile Science & Technolgoy, Shinshu University
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HASHIMOTO HAJIME
Department of Applied Biology, Faculty of Textile Science & Technology, Shinshu University
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Mitsui N
Asahimatsu Foods Corp. Nagano Jpn
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Shimosaka M
信州大・繊維・応生系
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Shimosaka Makoto
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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Okazaki M
Dep. Of Applied Biology Fac. Of Textile Sci. And Technol. Shinshu Univ.
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Okazaki Mitsuo
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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Hashimoto H
Institute For Production Research And Development The Nikka Whisky Co. Ltd.
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Chikara Ken-ichi
Department Of Applied Biology Faculty Of Textile Science & Technolgoy Shinshu University
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Yoshikawa Shigetoshi
Food Technology Research Institute Of Nagano Prefecture
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OKAZAKI MITSUO
Department of Applied Biology, Faculty of Textile Science & Technology, Shinshu University
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MITSUI NOBUO
Department of Applied Biology, Faculty of Textile Science & Technology, Shinshu University
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SHIMOSAKA MAKOTO
Department of Applied Biology, Faculty of Textile Science & Technology, Shinshu University
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HASHIMOTO HAJIME
Department of Applied Biology, Faculty of Textile Science & Technology, Shinshu University
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