Use of the PDR4 Gene as a Dominant Selective Marker in Combination with Cerulenin for Prototrophic Strains in Sacchromyces cerevisiae
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概要
- 論文の詳細を見る
We found that a 2.1-kbp KpnI fragment bearing the PDR4 (YAP1/PAR1/SNQ3) gene of Saccharomyces cerevisiae, conferring pleiotropic drug resistance to S. cerevisiae, confers resistance of the cells to 5 μg of cerulenin per ml when ligated into a multicopy vector and to 1 μg of cerulenin ligated into a single copy vector. This 2.1-kbp KpnI fragment lacks the C-terminal coding region of the published nucleotide sequence (Hussain, M. and Lenard, J. : Gene, 101,149-152,1991). This dosage-dependent cerulenin resistance induced by the 2.1-kbp KpnI fragment of PDR4 is useful as a dominant selective marker in genetic studies and in tranformation of prototrophic S. cerevisiae strains. We constructed a pair of tester strains for mating-type determination by introducing the multicopy plasmid bearing the 2.1-kbp KpnI fragment into a and α haploid strains of S. cerevisiae.
- 社団法人日本生物工学会の論文
- 1993-07-25
著者
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Harashima Satoshi
Department of Biotechnology, Graduate School of Engineering, Osaka University
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Harashima Satoshi
Department Of Biotechnology Faculty Of Engineering Osaka University
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Nakazawa Nobushige
Konishi Chuzo Co.
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Hashimoto H
Department Of Applied Biology Faculty Of Textile Science & Technology Shinshu University
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OSHIMA YASUJI
Department of Biotechnology, Faculty of Engineering, Osaka University
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HASHIMOTO HIDEAKI
Institute for Production Research and Development, Nikka Distilling Co.,
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Oshima Y
Faculty Of Engineering Kansai University
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Oshima Yasuji
Department Of Biotechnology Faculty Of Engineering Osaka University
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Hashimoto Hideaki
Institute For Production Research And Development Nikka Distilling Co.
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Oshima Yasuji
Department Of Biotechnology Faculty Of Engineering Kansai University
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