Tetraploid Formation through the Conversion of the Mating-type Alleles by the Action of Homothallic Genes in the Diploid Cells of Saccharomyces Yeasts
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概要
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Mating type conversiton by the homothallic genes in the diploid cells occured subsequent to the spontaneous or ultraviolet-light induced appearance of homozygosity of the mating-type alleles, a/a and α/α, from the a/α configuration. When homothallic, semi-homothallic or heterothallic a/α diploid cells were incubated with an excess population of a or α haploid cells having complementary nutritional markers in nutrient medium, prototrophic colonies appeard with a frequency of approximately 10^<-5> of the diploid cells on subsequent plating of the mating mixture on minimum medium. However, when a/α cells (irradiate with low dose of ultraviolet-light to accelerate the mitoric recombination) were directly plated on nutrient agar, and each colony appearing on the plate was tested for its mating response with the standard haploid cells, striking differences were observed depending on the genotypes for homothallism of the diplid cells. None of the 1,000 colonies so far tested of a perfect homothallic strain (the Ho type) showed mating reaction, while some isolated colonies of a heterothallic strain showed either a or α mating type activity. In the isolates showing mating potency from an Hp type semi-homothallic strain, solely α mating-type was observed, whereas in those from an Hq type semi-homothallic diplid showed solely a mating-type. These findings suggest that swiching of the a/α heterozygous configuration to a/a and α/α occurs in both heterothallic and homothallic cells. And the conversion of the a/a alleles to α/α ro vice versa, or from a/a and α/α to a/α depends on the genotype for homothallism of the cell. The former conversiton is followed by cell fusion between the converted and the unconverted cells to produce tetraploid cells. This inference was supported by tetrad analyses and determination of cell size and deoxyribonucleic acid contents of the cells of supposed tetraplid clones. Photomicrographic traces of cell fusion to produce tetraploid cells by the cultivation of a diploid ascospore derived from the supposed tetraploid clones also supported the above view.
- 公益社団法人日本生物工学会の論文
- 1977-01-25
著者
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Harashima Satoshi
Department Of Biotechnology Faculty Of Engineering Osaka University
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Harashima Satoshi
Department Of Fermentation Technology Osaka University
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Takano Isamu
Central Research Institute Of Suntory Ltd.
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Oshima Yasuji
Department Of Biotechnology Faculty Of Engineering Osaka University
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Oshima Yasuji
Department Of Fermentation Technology Osaka University
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OSHIMA TAKEHIRO
Central Research Institute of Suntory Ltd.
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Oshima Yasuji
Department Of Biotechnology Faculty Of Engineering Kansai University
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