One-Step Splitting of a Chromosome in Haploid Cells of Saccharomyces cerevisiae and Its Effect on the Cell Proliferation
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概要
- 論文の詳細を見る
A simple method has been developed for splitting a chromosome in Saccharomyces cerevisiae into two replicating halves. Haploid yeast cells of an ura3 mutant were transformed with a linear molecule bearing an S. cerevisiae DNA fragment for targeted integration into a chromosome, the URA3 and CEN4DNA sequences of S. cerevisiae, and a pair of inverted repeats (IRs) of the terminal 0.7 kbp of Tetrahymena ribosomal DNA (Tr). The integrated IRs of Tr ends of the molecule at a site on a chromosome in an ura3 mutant cell of S. cerevisiae subsequently resolved and the chromosome was split into two monocentric chromosomes in the Ura^+ transformant. Using this procedure, chromosomes XI, VIII, and II were each split into two halves, at the GCN3,YHR088,and VPS15 loci, respectively. Occurrence of the split chromosomes was confirmed by examination of their electrophoretic karyotypes. Some of the clones with the split chromosomes proliferated more quickly than the parental strain.
- 社団法人日本生物工学会の論文
- 1996-09-25
著者
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Harashima Satoshi
Department of Biotechnology, Graduate School of Engineering, Osaka University
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Widianto Donny
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Kim Kang-ho
Dept. Of Biotech. Osaka Univ.
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Harashima S
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Harashima Satoshi
Department Of Biotechnology Faculty Of Engineering Osaka University
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MUKAI YUKIO
Department of Biotechnology, Graduate School of Engineering, Osaka University
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Oshima Y
Faculty Of Engineering Kansai University
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Harashima Satoshi
Dept. Biotech. Grad. Sch. Eng. Osaka Univ.
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Mukai Yukio
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Mukai Yukio
Department Of Bioscience Faculty Of Bioscience Nagahama Institute Of Bio-science And Technology
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OSHIMA YASUJI
(Present address)Department of Biotechnology, Faculty of Engineering, Kansai University
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KIM KANG-HO
Department of Biotechnology, Faculty of Engineering, Osaka University
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HARASHIMA Satoshi
Department of Biotechnoiogy, Graduate School of Engineering, Osaka University
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