Saccharomyces cerevisiae protein phosphatase Ppz1 and protein kinases Sat4 and Ha15 are involved in the control of subcellular localization of Gln3 by likely regulating its phosphorylation state(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
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概要
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A Saccharomyces cerevisiae mutant lacking PPZ1, encoding a serine/threonine protein phosphatase (PPase), is caffeine-sensitive. To clarify the function of Ppz1 in resistance to caffeine, we attempted systematically to identify protein kinase (PKase) whose disruption lead to suppression of caffeine sensitive phenotype of the Δppz1 disruptant since disruption of PPZ1 might cause caffeine sensitivity by increasing its phosphorylated substrates and we presumed that disruption of genes for PKase sharing the substrate with Ppz1 could restore the resistance through bypassing necessity for dephosphorylation of substrates. Among the 102 viable pkase disruptions, disruption of either SAT4 or HAL5 suppressed the caffeine sensitivity phenotype and increased expression of ENA1, encoding a P-type ATPase of the Δppz1 disruptant. Because increased expression of ENA1 in the Δppz1 disruptant was found to be suppressed by disruption of GLN3, localization and phosphorylation of Gln3 in the Δppz1 disruptant was compared to that in the Δppz1Δsat4 and Δppz1Δhal5 double disruptants. Gln3 was found to accumulate in the nucleus in the Δppz1 disruptant, and this nuclear localization was abolished by disruption of either SAT4 or HAL5. Interestingly, the level of Gln3 phosphorylation in the Δppz1Δsat4 and Δppz1Δhal5 disruptants decreased relative to wild type independent of caffeine. From these observations, we conclude that Ppz1 controls Gln3 localization by regulating its phosphorylation state in combination with Sat4 and Hal5.
著者
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Hirasaki Masataka
Department of Biotechnology, Graduate School of Engineering, Osaka University
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Numamoto Minori
Department of Biotechnology, Graduate School of Engineering, Osaka University
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Sugiyama Minetaka
Department of Biotechnology, Graduate School of Engineering, Osaka University
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Kaneko Yoshinobu
Department of Biotechnology, Graduate School of Engineering, Osaka University
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Harashima Satoshi
Department of Biotechnology, Graduate School of Engineering, Osaka University
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Harashima S
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Harashima Satoshi
Department Of Biotechnology Faculty Of Engineering Osaka University
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Kaneko Y
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Kaneko Yoshinobu
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Nogi Yasuhisa
Department Of Molecular Biology Saitama Medical School
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Numamoto Minori
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Kaneko Yoshinobu
Dept. Biotech. Grad. Sch. Eng. Osaka Univ.
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Harashima Satoshi
Dept. Biotech. Grad. Sch. Eng. Osaka Univ.
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Horiguchi Masaya
Department of Biotechnology, Graduate School of Engineering, Osaka University
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Hirasaki Masataka
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Hirasaki Masataka
Department Of Bioresources Faculty Of Agriculture Ehime University
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Horiguchi Masaya
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Sugiyama M
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Sugiyama Minetaka
Department Of Biochemical Engineering & Science Kyushu Institute Of Technology
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Minetaka Sugiyama
Dept. Biotech. Grad. Sch. Eng. Osaka Univ.
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KANEKO Yoshinobu
Department of Biotechnoiogy, Graduate School of Engineering, Osaka University
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HARASHIMA Satoshi
Department of Biotechnoiogy, Graduate School of Engineering, Osaka University
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