Salt Shock Enhances the Expression of ZrATP2, the Gene for the Mitochondrial ATPase β Subunit of Zygosaccharomyces rouxii(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)

概要

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In the course of a study of cell wall proteins from the salt-tolerant yeast Zygosaccharomyces rouxii, a protein that increased its expression as the NaCl concentration of the culture medium increased was identified. Several degenerate primers were constructed based on partial amino acid sequences of this protein and were used in PCR amplification of a gene termed ZrATP2. The amino acid sequence deduced from nucleotide sequence of the gene revealed that ZrATP2 encodes the β subunit of mitochondrial F_1 ATPase. Northern blot analysis demonstrated that NaCl shock induced an elevation in ZrATP2 expression, which corresponded with the resumption of Z. rouxii cell growth after salt shock.
社団法人日本生物工学会の論文
2003-08-25