Effect of Vanadate on ATP-Induced Increase in Intracellular Calcium Ion Levels in Human Umbilical Vein Endothelial Cells
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概要
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We investigated the effect of ammonium vanadate (vanadate) on ATP-induced increases in intracellular calcium ion level ([Ca2+]i) of human umbilical vein endothelial cells (HUVEC) by fluorescence confocal microscopic imaging using the Ca2+-sensitive probe Calcium Green 1/AM. The ATP analogue 2-methylthio-ATP (2meS-ATP), at 10 μM, significantly increased the [Ca2+]i of HUVEC, and this was abolished by 1 μM thapsigargin (a calcium pump inhibitor), whereas extracellular free calcium had no effect. Vanadate at 10 μM also significantly increased the [Ca2+]i of HUVEC, which was abolished by 1 μM thapsigargin. However, vanadate at 1 μM did not exert such a significant effect. We thus examined the influence of ≤1 μM vanadate for 24 h on 2meS-ATP-induced increase in [Ca2+]i. Vanadate significantly reduced the action of 2meS-ATP at 1 μM but not at 0.1 μM. Endogenously released ATP is known to induce various actions on endothelial cells. The present results suggest that vanadate exerts a regulatory influence on the function of vascular endothelial cells.
著者
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Watanabe Yasuo
Department of Cardiology, Nihon University School of Medicine
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KUBOTA Yoko
Department of Biopharmaceutics, Nihon Pharmaceutical University
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KAGOTA Satomi
Department of Pharmacology, School of Pharmaceutical Sciences, Mukogawa Women's University
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SHINOZUKA Kazumasa
Department of Pharmacology, School of Pharmaceutical Sciences, Mukogawa Women's University
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TADA Yukari
Department of Pharmacology, School of Pharmacy and Pharmaceutical Sciences, Mukogawa Women's Univers
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NEJIME Namie
Department of Pharmacology, School of Pharmacy and Pharmaceutical Sciences, Mukogawa Women's Univers
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Shinozuka Kazumasa
Department Of Pharmacology Faculty Of Pharmaceutical Science Mukogawa Women's University
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Shibuichi Ikuo
Research & Development Headquarters, Asahi Soft Drinks Co., Ltd.
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Shinoda Yuki
Research & Development Headquarters, Asahi Soft Drinks Co., Ltd.
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Yamamoto Tomohiro
Research & Development Headquarters, Asahi Soft Drinks Co., Ltd.
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Kagota Satomi
Department Of Pharmacology Faculty Of Pharmaceutical Sciences Mukogawa Women's University
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Watanabe Yasuo
Department Of Agricultural Chemistry College Of Agriculture Ehime University
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Saito K
School Of Pharmacy And Pharmaceutical Sciences Mukogawa Women's University
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Tada Yukari
Department of Pharmacology, School of Pharmacy and Pharmaceutical Sciences, Mukogawa Women's University
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Shibuichi Ikuo
Research & Development Headquarters, Asahi Soft Drinks Co., Ltd.
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Shinozuka Kazumasa
Department of Pharmacology, School of Pharmacy and Pharmaceutical Sciences, Mukogawa Women's University
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Kubota Yoko
Department of Pharmacology, School of Medical Pharmaceutical Sciences, Nihon Pharmaceutical University
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Shinoda Yuki
Research & Development Headquarters, Asahi Soft Drinks Co., Ltd.
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Yamamoto Tomohiro
Research & Development Headquarters, Asahi Soft Drinks Co., Ltd.
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Kagota Satomi
Department of Pharmacology, School of Pharmacy and Pharmaceutical Sciences, Mukogawa Women's University
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Nejime Namie
Department of Pharmacology, School of Pharmacy and Pharmaceutical Sciences, Mukogawa Women's University
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Watanabe Yasuo
Department of Pharmacology, School of Medical Pharmaceutical Sciences, Nihon Pharmaceutical University
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Shinozuka Kazumasa
Department of Applied Chemistry, Faculty of Engineering, Yokohama National University
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Nejime Namie
Department of Pharmacology, School of Pharmaceutical Sciences, Mukogawa Women's University
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