Purification and Characterization of a Yeast Lytic β-1,3-Glucanase from Oerskovia xanthineolytica TK-1
スポンサーリンク
概要
- 論文の詳細を見る
A β-1,3-glucanase from the cultre supernatant of Oerskovia xanthineolytica TK-1 grown on minimal medium containing yeast glucan as the carbon source was purified to an electrophoretically homogeneous state by means of chromatography using DEAE-Sephacel, DEAE-Toyopearl 650 M and Bio-Gel P-2 column. The molecular mass was 40,000 Da and the pI was 6.5. The optimum pH was 7.5 and 5.5 when assayed on laminarin and yeast glucan, respectively. The enzyme had an affinity for yeast glucan and could lyse living yeast cells without the need for a second lytic component, an alkaline protease. Hydrolysis of yeast glucan was endolytic, yielding a mixture of glucose and biose. The N-terminal amino acid sequence of the enzyme showed no homology with that of a related β-1,3-glucanase from the same genus.
- 社団法人日本生物工学会の論文
- 1994-12-25
著者
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Watanabe Yasuo
Department of Cardiology, Nihon University School of Medicine
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Watanabe Yasuo
Department Of Cardiology Nihon University School Of Medicine
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Watanabe Y
Department Of Bioresources Faculty Of Agriculture Ehime University
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TAMAI YOUICHI
Department of Biological Resources, Faculty of Agriculture, Ehime University
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YAMAZAKI SHUJI
Department of Orthopaedic Surgery, Hokkaido University School of Medicine
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Iwata Junko
Department Of Bioresources Faculty Of Agriculture Ehime University
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Tamai Y
Department Of Bioresources Faculty Of Agriculture Ehime University
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Yamazaki S
Mie Univ. Tsu Jpn
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Tamai Youichi
Department Of Agricultural Chemistry College Of Agriculture Ehime University
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Watanabe Y
Department Of Cell Physiology Faculty Of Medicine Kagawa University
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SAEKI KAZUO
Department of Bioresource, Faculty of Agriculture, Ehime University
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Yamazaki Shuji
Department Of Bioresource Faculty Of Agriculture Ehime University
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Saeki Kazuo
Department Of Bioresources Faculty Of Agriculture Ehime University
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Watanabe Yasuo
Department Of Agricultural Chemistry College Of Agriculture Ehime University
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Watanabe Yasuo
Institute of Space and Astronautical Science
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