Role of the Carbohydrate Moiety in Phospholipase B from Torulaspora delbrueckii(Biological Chemistry)
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概要
- 論文の詳細を見る
Water-soluble phospholipase B containing approximately 50% carbohydrate, purified to homogeneity from yeast cell washings of Torulaspora delbrueckii, was treated with endoglycosidase H (Endo-H), and about 75% of the carbohydrate associated with this enzyme was removed. The native and carbohydrate-depleted enzymes were compared. The phospholipase B activity was higher in the carbohydrate-depleted enzyme than in the native form. The enzymatic activation by the removal of carbohydrate was due to the increased affinity of the enzyme for the substrate. The carbohydratedepleted phospholipase B was less stable upon incubation at 50℃ and more susceptible to proteolysis by V8 protease. These results suggested that the carbohydrate of phospholipase B in yeast cells stabilizes the enzyme conformation and protects the enzyme from proteolysis.
- 社団法人日本農芸化学会の論文
- 1990-03-23
著者
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Watanabe Yasuo
Department of Cardiology, Nihon University School of Medicine
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Watanabe Yasuo
Department Of Cardiology Nihon University School Of Medicine
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Watanabe Y
Department Of Bioresources Faculty Of Agriculture Ehime University
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TAMAI YOUICHI
Department of Biological Resources, Faculty of Agriculture, Ehime University
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Tamai Y
Department Of Bioresources Faculty Of Agriculture Ehime University
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MARUYAMA Masafumi
Department of Gastroenterology, Shinshu University School of Medicine
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Tamai Y
Ehime Univ. Ehime Jpn
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Tamai Youichi
Department Of Agricultural Chemistry College Of Agriculture Ehime University
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Watanabe Y
Department Of Cell Physiology Faculty Of Medicine Kagawa University
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KADOWAKI Hideki
Department of Bioresources, Faculty of Agriculture, Ehime University
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Maruyama M
Department Of Bioresources Faculty Of Agriculture Ehime University
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Maruyama Masafumi
Department Of Gastroenterology Shinshu University School Of Medicine
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Watanabe Y
Sonoda Gakuen Women's College
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Kadowaki H
Chiba Inst. Technol. Narashino Jpn
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Watanabe Yasuo
Department Of Agricultural Chemistry College Of Agriculture Ehime University
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Watanabe Yasuo
Institute of Space and Astronautical Science
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MARUYAMA Masafumi
Department of Bioresources, Faculty of Agriculture, Ehime University
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