Purification and Properties of Mannanase from Oerskovia xanthineolytica
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概要
- 論文の詳細を見る
A microorganism, identified as Oerskovia xanthineolytica, which is capable of growing on Saccharomyces cerevisiae mannan as a carbon source has been isolated. When grown on yeast mannan, the microorganism secretes an α-mannanase into the culture medium. The α-mannanase purified by chromatography with phenyl-Sepharose CL-4B, DEAE-Sephacel, and by gel-filtration on Sephacryl S-300 gave a single band on nondenaturing polyacrylamide gel electrophoresis and a single precipitation line with antibodies raised against the purified enzyme. The purified α-mannanase could be dissociated into several polypeptides depending on the denaturation conditions of protein. The molecular weight of the enzyme was estimated as over 1,500,000 and the sedimentation coefficient (S_<20,w>)was 16.4s. The enzyme showed optimal activity at pH between 7.0 to 8.0 and optimum temperature at 50℃. The enzyme seemed to be Ca^<2+>-dependent. The enzyme was activated by cysteine and sulfhydryl reagents. The enzyme was strongly inactivated by Zn^<2+>, Fe^<2+>, and Cu^<2+>, and completely inhibited by Ni^<2+>, Hg^<2+>, iodoacetic acid, EDTA, and cetyltrimetyl ammonium bromide. The purified enzyme hydrolyzed p-nitrophenyl α-mannopyranoside, α-1,2-mannopyranosyl-D-mannopyranose, and α-1,3-man-nopyranosyl-D-mannopyranose. The enzyme could also completely disintegrate the antigenic activities of yeast mannan prepared from S. cerevisiae.
- 社団法人日本生物工学会の論文
- 1990-10-25
著者
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Watanabe Y
Department Of Bioresources Faculty Of Agriculture Ehime University
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Tamai Y
Department Of Bioresources Faculty Of Agriculture Ehime University
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Watanabe Y
Department Of Cell Physiology Faculty Of Medicine Kagawa University
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SAEKI KAZUO
Faculty of Agriculture, Ehime University
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MIYASHITA YOUICHI
Faculty of Agriculture, Ehime University
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WATANABE YASUO
Faculty of Agriculture, Ehime University
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TAMAI YOUICHI
Faculty of Agriculture, Ehime University
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Saeki Kazuo
Department Of Bioresources Faculty Of Agriculture Ehime University
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Miyashita Youichi
Faculty Of Agriculture Ehime University
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Watanabe Yasuo
Institute of Space and Astronautical Science
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