An Activator Stimulating the Hydrolysis of Phospholipids by Phospholipase B from Torulaspora delbrueckii(Biological Chemistry)
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概要
- 論文の詳細を見る
An activator stimulating the enzymatic hydrolysis of phospholipids was purified to a homogeneous state from autolyzed Torulaspora delbrueckii cell washings. Autolyzed cell washings were extracted with chloroform and ethanol, and the activator was purified about 130-fold by sequential column chromatographies on DEAE-Sephacel, Sephacryl S-300, and TSK gel G 3000 SW (high performance liquid chromatography, HPLC). The molecular weight of the activator was about 175,000 as estimated by gel filtration on HPLC. However, the purified activator gave two protein bands corresponding to molecular weights from 102,000 to 129,000 and from 71,000 to 88,000, respectively, on SDS-polyacrylamide gel electrophoresis, when stained with silver stain reagent and periodic acid-Shiff (PAS) reagent. The activator was sensitive to heat treatment at 70℃ for 10 min. The purified activator had no enzymatic activity, but stimulated the hydrolysis of phospholipids by water-soluble and membrane-bound phospholipases B if the substrates were pre-incubated with the activator. No stimulation of hydrolysis by the enzyme was observed when the activator was pre-incubated with the enzyme. The hydrolytic rate of phosphatidylcholine by the enzyme at acidic pH (pH 2.6) depended upon the amount of activator added. On the other hand, the hydrolytic rate at alkaline pH (pH 7.6) was stimulated greatly by more than 0.04 nmol of the activator.
- 社団法人日本農芸化学会の論文
- 1989-07-23
著者
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Watanabe Y
Department Of Bioresources Faculty Of Agriculture Ehime University
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ABE Shunnosuke
Laboratory of Molecular Cell Biology, Faculty of Agriculture, Ehime University
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KUWABARA YUJI
Shikoku Milk Product Co. Ltd.
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Tamai Y
Department Of Bioresources Faculty Of Agriculture Ehime University
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Abe Shunnosuke
Department Of Bioresources Faculty Of Agriculture Ehime University
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Abe Shunnosuke
Laboratory Of Molecular Cell Biology Faculty Of Agriculture Ehime University
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TANAKA Seiji
Laboratory of Nuclear Science,Institute for Chemical Research,Kyoto University
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Tamai Youichi
Laboratory Of Biochemistry Faculty Of Agriculture Ehime University
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WATANABE Yasuo
Laboratory of Biochemistry, Faculty of Agriculture, Ehime University
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Watanabe Y
Department Of Cell Physiology Faculty Of Medicine Kagawa University
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KADOWAKI Hideki
Department of Bioresources, Faculty of Agriculture, Ehime University
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KUWABARA Yuji
Laboratory of Biochemistry, Faculty of Agriculture, Ehime University
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MARUYAMA Masarfumi
Laboratory of Biochemistry, Faculty of Agriculture, Ehime University
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KADOWAKI Hideki
Laboratory of Biochemistry, Faculty of Agriculture, Ehime University
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Tanaka S
Kyushu Univ. Fukuoka Jpn
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Maruyama M
Department Of Bioresources Faculty Of Agriculture Ehime University
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Kadowaki H
Chiba Inst. Technol. Narashino Jpn
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Watanabe Yasuo
Laboratory Of Biochemistry Faculty Of Agriculture Ehime University
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Tanaka Seiji
Laboratory Of Insect Life Cycles And Physiology National Institute Of Agrobiological Sciences At Ohw
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Tanaka Seiji
Laboratory Of Applied Zoology And Entomology Faculty Of Agriculture Ibaraki University
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Watanabe Yasuo
Institute of Space and Astronautical Science
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