Purification and Characterization of Phospholipase B from Candida utilis

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概要

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• Phospholipase B (PLB) from the asporogenous yeast Candida utilis was purified to homogeneity from a culture broth. The apparent molecular mass was 90–110 kDa by SDS–PAGE. The enzyme had two pH optima, one acidic (pH 3.0) and the other alkaline (pH 7.5). At acidic pH the enzyme hydrolyzed all phospholipids tested without metal ions. On the other hand, the PLB showed substrate specificity and required metal ions for alkaline activity.The cDNA sequence of the PLB was analyzed by a combination of several PCR procedures. The PLB encoded a protein consisting of 643 amino acids. The amino acid sequence contained a lipase consensus sequence (GxSxG) and catalytic arginine and aspartic acid motifs which were identified as the catalytic triad in the PLB from Kluyveromyces lactis, suggesting that the catalytic mechanism of the PLB is similar to that of cytosolic phospholipase A2 (cPLA2), found in mammalian tissues.

• 社団法人 日本農芸化学会の論文
• 2006-02-23

著者

• Tamai Youichi

  Laboratory Of Biochemistry Faculty Of Agriculture Ehime University

• Tamai Youichi

  Laboratory Of Food Biochemistry Department Of Bioresources Faculty Of Agriculture Ehime University

• WATANABE Yasuo

  Laboratory of Biochemistry, Faculty of Agriculture, Ehime University

• FUJINO Shuji

  Laboratory of Food Biochemistry, Department of Bioresources, Faculty of Agriculture, Ehime Universit

• AKIYAMA Daigo

  Laboratory of Food Biochemistry, Department of Bioresources, Faculty of Agriculture, Ehime Universit

• AKABOSHI Satoko

  Laboratory of Food Biochemistry, Department of Bioresources, Faculty of Agriculture, Ehime Universit

• FUJITA Tomonari

  Laboratory of Food Biochemistry, Department of Bioresources, Faculty of Agriculture, Ehime Universit

• Fujino Shuji

  Laboratory Of Food Biochemistry Department Of Bioresources Faculty Of Agriculture Ehime University

• Akiyama Daigo

  Laboratory Of Food Biochemistry Department Of Bioresources Faculty Of Agriculture Ehime University

• Fujita Tomonari

  Laboratory Of Food Biochemistry Department Of Bioresources Faculty Of Agriculture Ehime University

• Akaboshi Satoko

  Laboratory Of Food Biochemistry Department Of Bioresources Faculty Of Agriculture Ehime University

• Watanabe Yasuo

  Laboratory Of Biochemistry Faculty Of Agriculture Ehime University

• Watanabe Yasuo

  Laboratory Of Food Biochemistry Department Of Bioresources Faculty Of Agriculture Ehime University

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• Purification and Characterization of Phospholipase B from Candida utilis
• An Activator Stimulating the Hydrolysis of Phospholipids by Phospholipase B from Torulaspora delbrueckii(Biological Chemistry)
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• A Method for the Induction of Tumor-resistant Mice by Immunization with Diethyl Pyrocarbonate-treated Tumor Cells

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