Physicochemical and Immunochemical Properties of a Thermo-labile Antigen (TLA b) from the Yeast Cell Surface
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概要
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The physicochemical and immunochemical properties of a thermo-labile antigen (TLA b) which is located on the cell surface were studied. The sedimentation constant (S20, w), molecular weight with the sedimentation equilibrium method and intrinsic viscosity were 7.33S, 81, 000 and 0.0313 dl•g-1, respectively. TLA b was composed of two identical subunits on the basis of N-terminal amino acid analysis. The circular dichroic (CD) spectrum of the TLA b had a negative maximum at around 218nm. TLA b was estimated to have at least twelve antigenic d terminants by analysis of the quantitative precipitin reaction. The number of molecules of TLA b per cell wasestimated to be 5.65×106, this corresponded to 1.14% by weight of the fresh yeast.
著者
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Tamai Youichi
Department Of Agricultural Chemistry College Of Agriculture Ehime University
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Takakuwa Masayoshi
Department Of Agricultural Chemistry College Of Agriculture Ehime University
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SHINMOTO Hiroshi
Department of Agricultural Chemistry, Faculty of Agriculture, Ehime University
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