Purification and Some Properties of NAD(P)^+ Glycohydrolase from Saccharomyces cerevisiae : Studies on Autolysis of Pressed Baker's Yeast (V)
スポンサーリンク
概要
- 論文の詳細を見る
Two NAD(P)^+ glycohydrolase preparations were purified from pressed baker's yeast (Saccharomyces cerevisiae) which had been kept at 30℃ for 10 days. Purification was achieved by ammonium sulfate fractionation, followed by successive chromatographies on CM0-cellulose and Ultrogel AcA 44 and finally by electrofocusing. The purified enzymes were homogeneous on polyacrylamide gel electrophoresis and had molecular weights of 50,000 and 45,000,respectively. Both enzyme preparations exhibited almost identical enzymological properties and hydrolyzed NADP^+ as well as NAD^+. The enzyme acted on NAD^+ optimally at pH 5.5 and 50℃, and were stable between pH 3 and 11. The two enzyme preparations showed a high resistance to inhibition by nicotinamide.
- 公益社団法人日本生物工学会の論文
- 1982-04-25
著者
-
Mori Isao
(present Address)otsuka Assay Laboratory Otsuka Pharmaceuticals Co.
-
Takakuwa Masayoshi
Department Of Agricultural Chemistry College Of Agriculture Ehime University
-
Takakuwa Masayoshi
Department Of Agricultural Chemistry Faculty Of Agriculture Ehime University
-
YAMASAKI NOBUYUKI
(Present address)Laboratory of Biochemistry, Faculty of Agriculture, Kyushu University,
-
Yamasaki Nobuyuki
(present Address)laboratory Of Biochemistry Faculty Of Agriculture Kyushu University
関連論文
- Change of Lipid Composition of Zygosaccharomyces rouxii after Transfer to High Sodium Chloride Culture Medium : A Salt Tolerance of Yeast (II)
- Purification and Some Properties of Water-soluble Phospholipase B from Torulaspora delbrueckii(Biological Chemistry)
- Purification and Some Properties of NAD(P)^+ Glycohydrolase from Saccharomyces cerevisiae : Studies on Autolysis of Pressed Baker's Yeast (V)
- Depression of Synthesis of Linoleyl Residue in Zygosaccharomyces rouxii Cells by Sodium Chloride
- Physicochemical and Immunochemical Properties of a Thermo-labile Antigen (TLA b) from the Yeast Cell Surface
- Purification and Properties of a Thermo-labile Antigen from Bakers Yeast Cells
- Purification and Some Physicochemical Properties of Five Kinds of Thermo-labile Antigens from Yeast Cell Surface
- Studies on the Localization of Thermo-labile Protein Antigens in Yeast Cell Walls
- Degradation of Phospholipids and Liquefaction of Pressed Bakers Yeast by Acetic Acid Vapour
- Degradation of Cellular Phospholipids and Softening of Pressed Bakers Yeast
- Evidence for the Presence of Phospholipase A1 in Saccharomyces cerevisiae
- Cytotoxic Effects of Incubation in Acetate Buffer on MH134 Tumor Cells