Establishment of an Efficient Fermentation System of Gamma-Aminobutyric Acid by a Lactic Acid Bacterium, Enterococcus avium G-15, Isolated from Carrot Leaves
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概要
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In the present study, we successfully isolated a carrot leaf-derived lactic acid bacterium that produces gamma-aminobutyric acid (GABA) from monosodium L-glutamate (L-MSG) at a hyper conversion rate. The GABA-producing bacterium, identified as Enterococcus (E.) avium G-15, produced 115.7±6.4 g/l GABA at a conversion rate of 86.0±5.0% from the added L-MSG under the optimum culture condition by a continuous L-MSG feeding method using a jar-fermentor, suggesting that the bacterium displays a great potential ability for the commercial-level fermentation production of GABA. Using the reverse transcription polymerase chain reaction (RT-PCR) method, we analyzed the expression of genes for the GABA transporter and glutamate decarboxylase, designated gadT and gadG, respectively, which were cloned from the E. avium G-15 chromosome. Both genes were expressed even without the added L-MSG, but their expression was enhanced by the addition of L-MSG.
著者
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SUGIYAMA Masanori
Department of Fermentation Technology, Faculty of Engineering, Hiroshima University
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Kumagai Takanori
Department of Internal Medicine, Arita Kyouritsu Hospital, Japan
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NODA Masafumi
Department of Thoracic Surgery, institute of Development, Aging, and Cancer, Tohoku University
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Sugiyama Masanori
Department Of Fermentation Technology Faculty Of Engineering Hiroshima University
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Matoba Yasuyuki
Department of Molecular Microbiology and Biotechnology, Graduate School of Biomedical Sciences, Hiro
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Tamura Takayoshi
Department of Management and Systems Engineering Nagoya Institute of Technology
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Noda Masafumi
Department Of Artificial System And Engineering Chiba University
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MARUYAMA Masafumi
Department of Gastroenterology, Shinshu University School of Medicine
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Matoba Yasuyuki
Department Of Molecular Microbiology And Biotechnology Graduate School Of Biomedical Sciences Hirosh
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Ozaki Moeko
Department of Molecular Microbiology and Biotechnology, Graduate School of Biomedical Sciences, Hiro
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Maruyama Masafumi
Department Of Gastroenterology Shinshu University School Of Medicine
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Noda Masafumi
Department of Molecular Microbiology and Biotechnology, Graduate School of Biomedical Sciences, Hiroshima University
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Tamura Takayoshi
Department of Molecular Microbiology and Biotechnology, Graduate School of Biomedical Sciences, Hiroshima University
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MARUYAMA Masafumi
Department of Bioresources, Faculty of Agriculture, Ehime University
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Ozaki Moeko
Department of Molecular Microbiology and Biotechnology, Graduate School of Biomedical Sciences, Hiroshima University
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