Molecular Cloning and Functional Analysis of minD Gene from Streptomyces lavendulae ATCC25233(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
スポンサーリンク
概要
- 論文の詳細を見る
The minD gene encodes an inhibitor of cell division, which is involved in the regulation of the placement of the proper cell division site in rod-shaped bacteria. In the present study, we cloned the MinD homolog from the filamentous bacterium Streptomyces lavendulae ATCC25233. We showed that the protein exhibits ATPase activity and gives rise to a filamentous phenotype in Escherichia coli. However, the introduction of minD into Streptomyces lividans gave no apparent morphological alternation. We purified the Streptomyces minD gene product, expressed it in E. coli, and characterized it by enzyme kinetics. This is the first report characterizing the Streptomyces MinD homolog.
- 社団法人日本生物工学会の論文
- 2008-09-25
著者
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SUGIYAMA Masanori
Department of Fermentation Technology, Faculty of Engineering, Hiroshima University
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Kumagai Takanori
Department of Internal Medicine, Arita Kyouritsu Hospital, Japan
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Nguyen Tu
Department Of Mathematics University Of Hue
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Nguyen Tu
Department Of Molecular Microbiology And Biotechnology Graduate School Of Biomedical Sciences Hirosh
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Sugiyama Masanori
Department Of Fermentation Technology Faculty Of Engineering Hiroshima University
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Sugiyama Masanori
Dep. Of Molecular Microbiology And Biotechnology Graduate School Of Biomedical Sciences Hiroshima Un
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Sugiyama M
Department Of Molecular Microbiology And Biotechnology Graduate School Of Biomedical Sciences Hirosh
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Matoba Yasuyuki
Department of Molecular Microbiology and Biotechnology, Graduate School of Biomedical Sciences, Hiro
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Suzaki Toshinobu
Department of Biology, Graduate School of Science, Kobe University
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Matoba Yasuyuki
Dep. Of Molecular Microbiology And Biotechnology Graduate School Of Biomedical Sciences Hiroshima Un
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Matoba Yasuyuki
Department Of Molecular Microbiology And Biotechnology Graduate School Of Biomedical Sciences Hirosh
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Sugiyama Masanori
Department Of Molecular Microbiology And Biotechnology Hiroshima University
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Kumagai Takanori
Dep. Of Molecular Microbiology And Biotechnology Graduate School Of Biomedical Sciences Hiroshima Un
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Suzaki Toshinobu
Department Of Biology Graduate School Of Science Kobe University
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Suzaki Toshinobu
Department Of Biology Faculty Of Science Kobe University
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Sugiyama Masanori
Department Of Molecular Microbiology And Biotechnology Graduate School Of Biomedical Sciences Hirosh
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Sugiyama Masanori
Department of Molecular Microbiology and Biotechnology, Graduate School of Biomedical Sciences, Hiroshima University:Frontier Center for Microbiology, Hiroshima University
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