Ribosomal Resistance to Streptomycin in Strains Regenerated from Protoplasts of a Streptomycin-Susceptible Mutant Derived from a Streptomycin Producer

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When protoplasts of streptomycin-sensitive Streptomyces griseus KSN were regenerated to the mycelial form, streptomycin-resistant cultures appeared at a high frequency (approximately 1%). The resistance mechanism of these regenerated cultures was diferent from that of ancestral streptomycin-producing Streptomyces griseus HUT 6037,from which the parent S.griseus KSN was derived. While the resistance to streptomycin of S. griseus HUT 6037 was due mainly to streptomycin-phosphorylating activity of streptomycin 6-kinase produced in the cells, the streptomycin-resistant cultures regenerated from protoplasts produced no streptomycin 6-kinase but the ribosomes changed and became resistant to streptomycin. In highly streptomycin-resistant cultures regenerated, one of the 30S ribosomal proteins was altered in electrophoretic mobility as compared to the corresponding protein of S.griseus KSN.
社団法人日本生物工学会の論文
1983-08-25