Purification of the Early Sporulation Gene spo0F Product of Bacillus subtilis(Microbiology & Fermentaion Industry)
スポンサーリンク
概要
- 論文の詳細を見る
The RsaI fragment (750 base pairs) containing the entire early sporulation gene spo0F of Bacillus subtilis was inserted in the downstream region of the P_R promoter of the expression vector, pEBR-151. The recombinant plasmid thus obtained was introduced into Escherichia coli HB101 and the synthesis of the spo0F gene product was induced by a temperature shift up. After induction for 7hr, a protein of molecular weight 14,000 (14 K protein) was overproduced to about 9% of the total cellular protein. The 14 K protein was purified to 94% purity by four steps of column chromatography. Deletion analysis and the sequence determination of the NH_2-terminal amino acid residues of the purified 14 K protein confirmed that the 14 K protein is the spo0F gene product.
- 社団法人日本農芸化学会の論文
- 1986-11-23
著者
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KOBAYASHI YASUO
Department of Dentistry Oral Surgery, Nagano Municipal Hospital
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Oda Masanao
Department Of Applied Biochemistry Hiroshima University
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Nomi Ryosaku
Department Of Fermentation Technology Faculty Of Engineering Hiroshima University
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Nomi R
Department Of Fermentation Technology Hiroshima University
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Kawamura Fujio
Institute Of Applied Microbiology University Of Tokyo
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Kawamura Fujio
Institue Of Applied Microbiology The University Of Tokyo
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YOSHIKAWA Hirofumi
Institue of Applied Microbiology, The University of Tokyo
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Saito Hiuga
Institute Of Applied Microbiology University Of Tokyo
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Murooka Yoshikatsu
Department Of Fermentation Technology Hiroshima University
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Murooka Yoshikatsu
Depart. Of Bioteclnology Graduate School Of Eng. Osaka Univ.
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ISAKA Masami
Department of Applied Biochemistry, Hiroshima University
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Isaka Masami
Department Of Applied Biochemistry Hiroshima University
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Kobayashi Yasuo
Department Of Applied Biochemistry Hiroshima University
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Yoshikawa Hirofumi
Institute Of Applied Microbiology University Of Tokyo
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Kobayashi Yasuo
Department Of Anesthesia Asahikawa City Hospital
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