Isolation and Characterization of a Heavy Metal-Binding Protein from a Heavy Metal-Resistant Strain of Thiobacillus sp.

スポンサーリンク

概要

• 論文の詳細を見る

• A cadmium-inducible, high-molecular-weight, heavy metal-binding protein was isolated from cadmium-exposed cells of Thiobacillus sp. strain 13-1. The protein was purified by SDS-polyacrylamide gel electrophoresis, and extracted from the gel and renatured. The molecular weight of the protein was about 150 kDa and it bound 6.5,5.2,and 1.5 gram stoms of cadimum, copper, and zinc per mole of protein, respectively. The protein contained abnormal levels of histidine residues and low levels of cysteine residues. Together with the physicochemical characteristics of the protein, these results provide the first conclusive evidence for the existence of a novel type of heavy metal-binding protein in microorganisms.

• 社団法人日本生物工学会の論文
• 1993-07-25

著者

• YOSHIDA Naoto

  Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, Miyazaki University

• MUROOKA YOSHIKATSU

  Department of Agricultural Chemistry, Faculty of Bioresources, Mie University, Department of Biotech

• Murooka Yoshikatsu

  Department Of Biotechnology Graduate School Of Engineering Osaka University:hiroshima Institute Of T

• Murooka Y

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Murooka Yoshikatsu

  Department Of Fermentation Technology Faculty Of Engineering Hiroshima University

• Morinaga T

  Faculty Of Life And Environmental Science Prefectural University Of Hiroshima

• Morinaga T

  Hiroshima Univ. Higashi‐hiroshima Jpn

• Morinaga Tsutomu

  Department Of Fermentation Technology Faculty Of Engineering Hiroshima University

• Morinaga Tsutomu

  Faculty Of Life And Environmental Science Prefectural University Of Hiroshima

• Yoshida Naoto

  Department Of Biochemistry And Applied Biosciences Faculty Of Agriculture Miyazaki University

• Morinaga Tsutomu

  Department Of Bioresource Development Hiroshima Prefectural University

• Yoshida N

  Department Of Biochemistry And Applied Biosciences Faculty Of Agriculture Miyazaki University

• Murooka Yoshikatsu

  Depart. Of Bioteclnology Graduate School Of Eng. Osaka Univ.

• Yoshida Naoto

  Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University

関連論文

• Characterization of the C-terminal truncated form of amylopullulanase from Lactobacillus plantarum L137(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
• Purification and Characterization of Laminaran Hydrolases from Trichoderma viride(Microbiology & Fermentation Technology)
• Structural Characterization of a New Acidic Ecopolysaccharide and Cyclic (1→2) β-Glucan Produced by Rhizobium huakuii Forming Nodules on Astragalus sinicus
• Quantification of relative flying fish paste content in the processed seafood ago-noyaki using real-time PCR
• Characterization of Gene Encoding Amylopullulanase from Plant-Originated Lactic Acid Bacterium, Lactobacillus plantarum L137(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
• Promotion of Metal Accumulation in Nodule of Astragalus sinicus by the Expression of the Iron-regulated Transporter Gene in Mesorhizobium huakuii subsp. rengei B3(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
• Production of Vitamin B_ in Genetically Engineered Propionibacterium freudenreichii(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
• Molecular Analysis of Promoter Elements from Propionibacterium freudenreichii (GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
• Heterologous Expression of a Gene Encoding Cholesterol Oxidase in Probiotic Strains of Lactobacillus plantarum and Propionibacterium freudenreichii under the Control of Native Promoters
• Development of a Host-Vector System for Lactobacillus plantarum L137 Isolated from a Traditional Fermented Food Produced in the Philippines
• Characterization of the hemB Gene Encoding δ-aminolevulinic Acid Dehydratase from Propionibacterium freudenreichii
• Use of lac Gene Fusions to Study Regulation of Tyramine Oxidase, Which is Involved in Derepression of Latent Arylsulfatase in Escherichia coli
• In Vivo Cloning of Arylsulfatase-Tyramine Oxidase Genes in rec Strains of Klebsiella aerogenes
• 2P157 アクトミオシンによる微小検出システム構築の試み(筋肉(筋蛋白質・収縮),第48回日本生物物理学会年会)
• Secretory Production of Ascorbate Oxidase by Cultured Cells of Cucumber
• Fungal succession in the early decomposition process of pine cones on the floor of Pinus densiflora forests
• Characterization and Identification of Bacterial Strains Isolated from Corroded Concrete in the Accumulation Stratum and Their Resistance Levels to Heavy Metals
• Isolation and Characterization of a Heavy Metal-Binding Protein from a Heavy Metal-Resistant Strain of Thiobacillus sp.
• Resistance to and Uptake of Heavy Metals in Mushrooms
• Authentication of flying-fish-meal content of processed food using PCR-RFLP
• Complete mitochondrial DNA sequence of the Japanese flying fish Cypselurus hiraii
• Photoregulated tyrosinase gene in Polyporus arcularius
• Agrobacterium tumefaciens-mediated genetic transformation of the white root rot ascomycete Rosellinia necatrix
• Heterothallic life cycle in the white root rot fungus Rosellinia necatrix
• Molecular Cloning of Three Genes Encoding G Protein Alpha Subunits in the White Root Rot Fungus, Rosellinia necatrix
• 711 Bioremediation system for heavy metals using leguminous plant and Rhizobia symbiosis
• Lipase Production of a New Thermophilic Fungus, Humicola lanuginosa var. catenulata
• Molecular Cloning and Characterization of Two cDNAs Encoding Asparagine Synthetase from Astragalus sinicus Nodules
• Cloning and alaysis of a nodulin gene which encodes protease of Astragalus sinicus (Renge-sou)
• Cellulase Productivity of Eupenicillium javanicum : Studies on the Re-utilization of Cellulosic Resources (V).
• Alternation of nuclear phase in the filamentous basidiomycete, Helicobasidium mompa
• Enterobater colacae A105,Isolated from the Surface of Root Nodules of Astragalus sinicus cv. Japan, Stimulates Nodulation by Rhizobium huakuii bv. renge
• Formation of Root Nudules by Rhizobium huakuii biovar. renge bv. nov. on Astragalus sinicus cv. Japan
• Partial contig map of the smallest chromosome in Coprinus cinereus
• Purification, Characterization, and Crystallization of Monoamine Oxidase from Escherichia coli K-12
• Nucleotide Sequence of the Gene for Monoamine Oxidase (maoA) from Escherichia coli
• 3P-109 アクトミオシンによる微小検出システム構築の試み(筋肉(筋蛋白質・収縮),第47回日本生物物理学会年会)
• Characterization and Sequencing of the Region Required for Replication of a Non-Selftransmissible Plasmid pEC3 Isolated from Erwinia carotovora subsp. carotovora
• Difference in Thermal Degradation Behavior of ZrO_2 and HfO_2 Anodized Capacitors
• Transformation of Dimethyl Sulfide and Related Compounds by Cultures and Cell Extracts of Marine Phytoplankton
• Toward Elucidating the Full Spectrum of Mite Allergens : State of the Art
• Accumulation of Compatible Solutes, Ectoine and Hydroxyectoine, in a Moderate Halophile, Halomonas elengata KS3 Isolated from Dry Salty Land in Thailand
• Primary Structure of Cytochrome c Gene from the White Root Rot Fungus Rosellinia necatrix(Biochemistry & Molecular Biology)
• Variation of the Amino Acid Content of Arabidopsis Seeds by Expressing Soybean Aspartate Aminotransferase Gene
• Isolation, Characterization, and Molecular Cloning of a Thermostable Xylitol Oxidase from Streptomyces sp.IKD472
• Root Hair Deformation of Symbiosis-Deficient Mutants of Lotus japonicus by Application of Nod Factor from Mesorhizobium loti
• Cyclic AMP Regulation of the mob Operon of the Non-Self-Transmissible Plasmid pEC3 Isolated from a Phytopathogenic Bacterium
• Recent Advances in Studies on Streptomyces Cholesterol Oxidase and Gene Clusters Involved in Steroid Catabolism in Prokaryotes
• Genetic Modification of the Streptomyces Cholesterol Oxidase Gene for Expression in Escherichia coli and Development of Promoter-Probe Vectors for Use in Enteric Bacteria
• Purification and Characterization of the 3-Ketosteroid-Δ^1-Dehydrogenase of Arthrobacter simplex Produced in Streptomyces lividans^1
• Secretory Overproduction of Streptomyces Cholesterol Oxidase by Streptomyces lividans with a Multi-Copy Shuttle Vector
• Phylogenetic Position of Mesorhizobium huakuii subsp.rengei, a Symbiont of Astragalus sinicus cv.Japan
• 1056 Intraspesific variation of Mesorhizobium strains isolated from Astragalus sinicus cv. Japan
• AsNODc22, a Novel Nodulin Gene of Astragalus sinicus, Encodes a Protein that Localizes along the Cell Wall of Bacteria-Infected Cells in a Nodule
• Phylogeny of Bacterial Symbionts of the Laguminous Tree Acacia mangium
• 235 Phylogeny of rhizobia isolated from nodules on Acacia mangium through RFLP analyses of the 16s rDNA and Repetitive Extragenic Palindromic sequence.
• Grouping of Bradyrhizobium USDA Strains by Sequence Analysis of 16S rDNA and 16S-23S rDNA Internal Transcribed Spacer Region(Soil Biology)
• Identification of Cholesterol Oxidase from Fast-Growing Mycobacterial Strains and Rhodococcus sp.
• Origin of Fan Palm (Livistona chinensis R. Br. var. subglobosa Becc.) in Aoshima, Japan
• The Monoamine Regulon Including Syntheses of Arylsulfatase and Monoamine Oxidase in Bacteria
• Heavy Metal Particle Resistance in Thiobacillus intermedius 13-1 Isolated from Corroded Concrete
• Purification and Properties of Betaine Aldehyde Dehydrogenase from Xanthomonas translucens
• Effect of nutrient salts eluted from hedoro blocks on growth and survival rate of gametophyte and juvenile Eisenia bicyclis
• ROPME海域における海中照度分布およびオイルスリックによる光減衰
• Uniaxial compression strength and erosive potential rate of rocks in strata on the coast of Miura Peninsula with specific relation to the algal communities of Eisenia bicyclis and Ecklonia cava
• Root Hair Deformation of Symbiosis-Deficient Mutants of Lotus japonicus by Application of Nod Factor from Mesorhizobium loti
• Expression of a Major House Dust Mite Allergen Gene from Dermatophagoides farinae in Lotus japonicus Accession Miyakojima MG-20 (Plant Biotechnology)
• Construction of an Additional Metal-Binding Site in Human Metallothionein-2(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
• Interactions of Arsenic with Human Metallothionein-2
• Hybridization and Breeding of the Benomyl Resistant Mutant, Trichoderma harzianum Antagonized to Phytopathogenic Fungi by Protoplast Fusion
• Chrysotile Fibers Penetrate Escherichia coli Cell Membrane and Cause Cell Bursting by Sliding Friction Force on Agar Plates (ENVIRONMENTAL BIOTECHNOLOGY)
• Behavior of chromosomes after meiosis in Coprinus cinereus
• Inheritance of chromosome length polymorphisms in Coprinus cinereus
• Purification and Characterization of Monoamine Oxidase from Klebsiella aerogenes
• Effect of Hypergravitational Stress on Microbial Cell Viability
• Toxicity of Cadmium Particle Dust in Bacterial Cells
• A Simple PCR Method for Distinguishing Saccharomyces cerevisiae from Its Sibling species by Amplification of the RPL2 Region
• Root Hair Abundant Genes LjRH101 and LjRH102 Encode Peroxidase and Xyloglucan Endotransglycosylase in Lotus japonicus(Plant Biotechnology)
• 懸濁無機粒子の濃度,粒径および種類がアサリの餌捕捉へ与える影響
• Analytical Methods for Quantification of Relative Flying Fish Paste Content in Processed Sea Food (ago-noyaki)
• Production of 5-aminolevulinic acid by Propionibacterium acidipropionici TISTR442(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
• Agrobacterium tumefaciens-mediated transformation of the violet root-rot fungus, Helicobasidium mompa, and the effect of activated carbon
• Pollen Development and Tube Growth are Affected in the Symbiotic Mutant of Lotus japonicus, crinkle
• Gene Targeting in a Klebsiella sp. by Fusaric Acid Selection and the Use of Temperature Sensitive Replicon
• Nucleotide Sequences of BstUI Repetitive Elements from Nuclear DNA of Chlorella ellipsoidea C-87
• Rhodobacter sphaeroides Mutants Which Accumulate 5-Aminolevulinic Acid under Aerobic and Dark Conditions
• Purification of the Early Sporulation Gene spo0F Product of Bacillus subtilis(Microbiology & Fermentaion Industry)
• Genetic and Protein Engineering of Diagnostic Enzymes, Cholesterol Oxidase and Xylitol Oxidase
• Cellulose and Xylan Degrading Enzymes of the Plant Pathogenic Fungus, Fusarium oxyspomm SUF850(Microbiology & Fermentation Industry)
• Adsorption of Bacterial Cells to Crystal Particles of Heavy Metals : Role of Electrostatic Interaction
• Nucleotide Sequence Analysis of a Region Upstream of the Cholesterol Oxidase-Cytochrome P450 Operon of Streptomyces sp. SA-COO Revealing Repeating Units Coding for Putative Transmembrane and DNA-Binding Proteins
• Efficient Transformation of Mesorhizobium huakuii subsp.rengei and Rhizobium Species
• 167 Bioremediation of heavy metals by expression of the metallothionein in Renge-soh nodules
• Genetic Design of a Gene for Human Metallothionein II and Its Expression as an Active Fusion Protein in Escherichia coli
• Molecular cloning and sequence analysis of the glyceraldehyde-3-phosphate dehydrogenase gene from the violet root rot fungus, Helicobasidium mompa
• Amino Acid Residues Specific for the Catalytic Action towards α-1,6-Glucosidic Linkages in Klebsiella Pullulanase
• 562 "Guided Genomic" Walking", a Systematic Approach to Clone Intermediate-Sized Restriction fragments Extending Outside the Boundaries of Known Sequences
• Prophylactic effect of Lactobacillus oral vaccine expressing a Japanese cedar pollen allergen(MEDICAL BIOTECHNOLOGY)
• Genetic Manipulation System in Propionibacteria
• Prophylactic effect of Lactobacillus oral vaccine expressing a Japanese cedar pollen allergen

もっと見る 閉じる

スポンサーリンク