Secretory Production of Ascorbate Oxidase by Cultured Cells of Cucumber

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概要

• 論文の詳細を見る

• Peelings of cucumber (Cucumis sativus) were induced to form callus, which in turn produced ascorbate oxidase, on Murashige-Skoog basal medium with plant hormones. Cell lines which produced high levels of ascorbate oxidase and had a high growth rate were selected. Addition of 100 times more copper, a prosthetic metal of ascorbate oxidase, than that in the basal medium resulted in a signification increase in enzyme activity. A suspension culture of the cells was also established, which was found to produce ascorbate oxidase in the medium. The secretion of the enzyme from the cells was stimulated by addition of calcium ions. Maximum cell yield and maximum levels of ascorbate oxidase were obtained at about the 20th day by shaking culture, but similar growth and enzyme activity were found at the 10th day in an air-lift type jar fermentor.

• 社団法人日本生物工学会の論文
• 1989-09-25

著者

• MUROOKA YOSHIKATSU

  Department of Agricultural Chemistry, Faculty of Bioresources, Mie University, Department of Biotech

• Murooka Yoshikatsu

  Department Of Fermentation Technology Faculty Of Engineering Hiroshima University

• Aimi T

  Hiroshima City Univ. Hiroshima Jpn

• Aimi Tadanori

  Department Of Biochemistry And Biotechnology Faculty Of Agriculture Tottori University

• CHO HYEON-JE

  Department of Fermentation Technology, Faculty of Engineering, Hiroshima University

• PAIK SOON-YOUNG

  Department of Fermentation Technology, Faculty of Engineering, Hiroshima University

• Cho H‐j

  Hiroshima Univ. Higashi‐hiroshima Jpn

• Cho Hyeon-je

  Department Of Fermentation Technology Faculty Of Engineering Hiroshima University

• Paik Soon-young

  Department Of Fermentation Technology Faculty Of Engineering Hiroshima University

• Murooka Yoshikatsu

  Depart. Of Bioteclnology Graduate School Of Eng. Osaka Univ.

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