Genetic Manipulation System in Propionibacteria

スポンサーリンク

概要

• 論文の詳細を見る

• Members of the genus Propionibacterium are widely used in the production of vitamin B_<12>, tetrapyrrole compounds, and propionic acid as well as in probiotic and cheese industries. Shuttle vectors were developed in propionibacteria using replicons from endogenous plasmids in Propionibacterium and Escherichia coli and an appropriate selection marker. The efficient transformation was achieved using the shuttle vector prepared from Propionibacterium freudenreichii to overcome the high restriction modification system in propionibacteria. Expression vectors with native promoters for use in propionibacteria were also developed. Using this system, cholesterol oxidase, which is used as a diagnostic enzyme, was produced in P.freudenreichii. Genes involved in 5-aminolevulinic acid (ALA) and vitamin B_<12> biosynthesis in propionibacteria were isolated. ALA in propionibacteria could be synthesized via both the C4 pathway (condensation of glycine and succinyl CoA) and the C5 pathway (from glutamate). The hemA gene encoding ALA synthase from Rhodobacter spheroides, was overexpressed and ALA accumulated in P.freudenreichii. Thus, the genetic manipulation systems in propionibacteria will facilitate genetic studies of probiotics and the vitamin B_<12> biosynthetic pathway.

• 公益社団法人日本生物工学会の論文
• 2002-01-25

著者

• Kiatpapan Pornpimon

  Biochemistry Unit School Of Science Rangsit University

• Murooka Yoshikatsu

  Depart. Of Bioteclnology Graduate School Of Eng. Osaka Univ.

関連論文

• Characterization of the C-terminal truncated form of amylopullulanase from Lactobacillus plantarum L137(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
• Structural Characterization of a New Acidic Ecopolysaccharide and Cyclic (1→2) β-Glucan Produced by Rhizobium huakuii Forming Nodules on Astragalus sinicus
• Characterization of Gene Encoding Amylopullulanase from Plant-Originated Lactic Acid Bacterium, Lactobacillus plantarum L137(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
• Promotion of Metal Accumulation in Nodule of Astragalus sinicus by the Expression of the Iron-regulated Transporter Gene in Mesorhizobium huakuii subsp. rengei B3(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
• Production of Vitamin B_ in Genetically Engineered Propionibacterium freudenreichii(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
• Molecular Analysis of Promoter Elements from Propionibacterium freudenreichii (GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
• Heterologous Expression of a Gene Encoding Cholesterol Oxidase in Probiotic Strains of Lactobacillus plantarum and Propionibacterium freudenreichii under the Control of Native Promoters
• Development of a Host-Vector System for Lactobacillus plantarum L137 Isolated from a Traditional Fermented Food Produced in the Philippines
• Characterization of the hemB Gene Encoding δ-aminolevulinic Acid Dehydratase from Propionibacterium freudenreichii
• Use of lac Gene Fusions to Study Regulation of Tyramine Oxidase, Which is Involved in Derepression of Latent Arylsulfatase in Escherichia coli
• In Vivo Cloning of Arylsulfatase-Tyramine Oxidase Genes in rec Strains of Klebsiella aerogenes
• Secretory Production of Ascorbate Oxidase by Cultured Cells of Cucumber
• Characterization and Identification of Bacterial Strains Isolated from Corroded Concrete in the Accumulation Stratum and Their Resistance Levels to Heavy Metals
• Isolation and Characterization of a Heavy Metal-Binding Protein from a Heavy Metal-Resistant Strain of Thiobacillus sp.
• Resistance to and Uptake of Heavy Metals in Mushrooms
• 711 Bioremediation system for heavy metals using leguminous plant and Rhizobia symbiosis
• Molecular Cloning and Characterization of Two cDNAs Encoding Asparagine Synthetase from Astragalus sinicus Nodules
• Enterobater colacae A105,Isolated from the Surface of Root Nodules of Astragalus sinicus cv. Japan, Stimulates Nodulation by Rhizobium huakuii bv. renge
• Formation of Root Nudules by Rhizobium huakuii biovar. renge bv. nov. on Astragalus sinicus cv. Japan
• Purification, Characterization, and Crystallization of Monoamine Oxidase from Escherichia coli K-12
• Nucleotide Sequence of the Gene for Monoamine Oxidase (maoA) from Escherichia coli
• Characterization and Sequencing of the Region Required for Replication of a Non-Selftransmissible Plasmid pEC3 Isolated from Erwinia carotovora subsp. carotovora
• Transformation of Dimethyl Sulfide and Related Compounds by Cultures and Cell Extracts of Marine Phytoplankton
• Toward Elucidating the Full Spectrum of Mite Allergens : State of the Art
• Accumulation of Compatible Solutes, Ectoine and Hydroxyectoine, in a Moderate Halophile, Halomonas elengata KS3 Isolated from Dry Salty Land in Thailand
• Variation of the Amino Acid Content of Arabidopsis Seeds by Expressing Soybean Aspartate Aminotransferase Gene
• Isolation, Characterization, and Molecular Cloning of a Thermostable Xylitol Oxidase from Streptomyces sp.IKD472
• Root Hair Deformation of Symbiosis-Deficient Mutants of Lotus japonicus by Application of Nod Factor from Mesorhizobium loti
• Cyclic AMP Regulation of the mob Operon of the Non-Self-Transmissible Plasmid pEC3 Isolated from a Phytopathogenic Bacterium
• Recent Advances in Studies on Streptomyces Cholesterol Oxidase and Gene Clusters Involved in Steroid Catabolism in Prokaryotes
• Genetic Modification of the Streptomyces Cholesterol Oxidase Gene for Expression in Escherichia coli and Development of Promoter-Probe Vectors for Use in Enteric Bacteria
• Purification and Characterization of the 3-Ketosteroid-Δ^1-Dehydrogenase of Arthrobacter simplex Produced in Streptomyces lividans^1
• Secretory Overproduction of Streptomyces Cholesterol Oxidase by Streptomyces lividans with a Multi-Copy Shuttle Vector
• Phylogenetic Position of Mesorhizobium huakuii subsp.rengei, a Symbiont of Astragalus sinicus cv.Japan
• AsNODc22, a Novel Nodulin Gene of Astragalus sinicus, Encodes a Protein that Localizes along the Cell Wall of Bacteria-Infected Cells in a Nodule
• Phylogeny of Bacterial Symbionts of the Laguminous Tree Acacia mangium
• Identification of Cholesterol Oxidase from Fast-Growing Mycobacterial Strains and Rhodococcus sp.
• Origin of Fan Palm (Livistona chinensis R. Br. var. subglobosa Becc.) in Aoshima, Japan
• The Monoamine Regulon Including Syntheses of Arylsulfatase and Monoamine Oxidase in Bacteria
• Heavy Metal Particle Resistance in Thiobacillus intermedius 13-1 Isolated from Corroded Concrete
• Root Hair Deformation of Symbiosis-Deficient Mutants of Lotus japonicus by Application of Nod Factor from Mesorhizobium loti
• Expression of a Major House Dust Mite Allergen Gene from Dermatophagoides farinae in Lotus japonicus Accession Miyakojima MG-20 (Plant Biotechnology)
• Construction of an Additional Metal-Binding Site in Human Metallothionein-2(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
• Interactions of Arsenic with Human Metallothionein-2
• Purification and Characterization of Monoamine Oxidase from Klebsiella aerogenes
• A Simple PCR Method for Distinguishing Saccharomyces cerevisiae from Its Sibling species by Amplification of the RPL2 Region
• Root Hair Abundant Genes LjRH101 and LjRH102 Encode Peroxidase and Xyloglucan Endotransglycosylase in Lotus japonicus(Plant Biotechnology)
• Production of 5-aminolevulinic acid by Propionibacterium acidipropionici TISTR442(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
• Pollen Development and Tube Growth are Affected in the Symbiotic Mutant of Lotus japonicus, crinkle
• Gene Targeting in a Klebsiella sp. by Fusaric Acid Selection and the Use of Temperature Sensitive Replicon
• Nucleotide Sequences of BstUI Repetitive Elements from Nuclear DNA of Chlorella ellipsoidea C-87
• Rhodobacter sphaeroides Mutants Which Accumulate 5-Aminolevulinic Acid under Aerobic and Dark Conditions
• Purification of the Early Sporulation Gene spo0F Product of Bacillus subtilis(Microbiology & Fermentaion Industry)
• Genetic and Protein Engineering of Diagnostic Enzymes, Cholesterol Oxidase and Xylitol Oxidase
• Adsorption of Bacterial Cells to Crystal Particles of Heavy Metals : Role of Electrostatic Interaction
• Nucleotide Sequence Analysis of a Region Upstream of the Cholesterol Oxidase-Cytochrome P450 Operon of Streptomyces sp. SA-COO Revealing Repeating Units Coding for Putative Transmembrane and DNA-Binding Proteins
• Efficient Transformation of Mesorhizobium huakuii subsp.rengei and Rhizobium Species
• 167 Bioremediation of heavy metals by expression of the metallothionein in Renge-soh nodules
• Genetic Design of a Gene for Human Metallothionein II and Its Expression as an Active Fusion Protein in Escherichia coli
• Amino Acid Residues Specific for the Catalytic Action towards α-1,6-Glucosidic Linkages in Klebsiella Pullulanase
• 562 "Guided Genomic" Walking", a Systematic Approach to Clone Intermediate-Sized Restriction fragments Extending Outside the Boundaries of Known Sequences
• Prophylactic effect of Lactobacillus oral vaccine expressing a Japanese cedar pollen allergen(MEDICAL BIOTECHNOLOGY)
• Genetic Manipulation System in Propionibacteria
• Prophylactic effect of Lactobacillus oral vaccine expressing a Japanese cedar pollen allergen
• DNA Synthesis and Fragmentation in Bacteroids during Astragalus sinicus Root Nodule Development

もっと見る 閉じる

スポンサーリンク