Cyclic AMP Regulation of the mob Operon of the Non-Self-Transmissible Plasmid pEC3 Isolated from a Phytopathogenic Bacterium
スポンサーリンク
概要
- 論文の詳細を見る
A non-self-transmissible multiple-copy plasmid, pEC3, isolated from the phytopathogenic bacterium, Erwinia carotovora subsp.carotovora, can be mobilized with the help of a self-transmissible plasmid. When donors carrying a cya or a crp mutation were used, the mobilization efficiencies of pEC3 were markedly decreased. The covalently closed circular conformation of plasmid DNA containing both oriT and mob genes of pEC3 was found to be relaxed after the addition of cyclic AMP(cAMP). The relaxed DNA contained a specific single-strand DNA nick within the oriT region. RNA transcription of the mob operon was also increased by the addition of cAMP. These results indicate that the expression of the mob operon of pEC3 is positively regulated by cAMP and that the mob gene products are involved in nicking within the oriT region.
- 社団法人日本生物工学会の論文
- 1998-12-25
著者
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NOMURA NOBUHIKO
Institute of Life and Environmental Sciences, University of Tsukuba
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YAMASHITA MITSUO
Department of Agricultural Chemistry, Faculty of Bioresources, Mie University, Department of Biotech
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MUROOKA YOSHIKATSU
Department of Agricultural Chemistry, Faculty of Bioresources, Mie University, Department of Biotech
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Yamashita Mitsuo
Department Of Biotechnology Graduate School Of Engineering Osaka University:department Of Applied Ch
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Yamashita M
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Murooka Yoshikatsu
Department Of Biotechnology Graduate School Of Engineering Osaka University:hiroshima Institute Of T
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Murooka Y
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Nomura Nobuhiko
Institute Of Applied Biochemistry University Of Tsukuba
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Murooka Yoshikatsu
Depart. Of Bioteclnology Graduate School Of Eng. Osaka Univ.
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Nomura N
Institute Of Applied Biochemistry University Of Tsukuba
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Matsumoto Yukimasa
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Yamashita Mitsuo
Department Of Agricultural Chemistry Faculty Of Bioresources Mie University Department Of Biotechnol
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