Participation of Nitrite Reductase in Conversion of NO_2^- to NO_3^- in a Heterotrophic Nitrifier, Burkholderia cepacia NH-17, with Denitrification Activity
スポンサーリンク
概要
- 論文の詳細を見る
The metabolic characteristics of the NO_2^- transforming activities of Burkholderia cepacia NH-17, which was isolated as a heterotrophic nitrifying bacterium with O_2 tolerant denitrification activity, were characterized. The conversion of NO_2^- to N_2O and NO_3^- occurred concomitantly with a decrease in NO_2^- under aerobic conditions in growing cultures. In an in vivo assay, production of N_2O and NO_3^- was induced by NO2〜 as an inducer for denitrification, and nitrite reductase activity in sonicated fraction (NiR) assay indicated that in vitro nitrite reductase activity was also induced by NO_2^-. These results suggested that nitrification and denitrification in Burkholderia cepacia NH-17 might be closely related. Therefore, we constructed a nirS knockout mutant of Burkholderia cepacia NH-17. The mutant had no in vitro nitrite reductase activity and did not convert NO_2^- to N_2O and NC_3^-. These properties were restored by introducing the intact nirS gene into the mutant strain, indicating that reduction of NO_2^- to NO is necessary for the conversion of NO_2〜 to NO_3^- in Burkholderia cepacia NH-17.
- 日本微生物生態学会の論文
著者
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UCHIYAMA HIROO
Institute of Life and Environmental Sciences, University of Tsukuba
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NOMURA NOBUHIKO
Institute of Life and Environmental Sciences, University of Tsukuba
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Nakajima Toshiaki
Institute Of Applied Biochemistry University Of Tsukuba
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Nakajima-kambe Toshiaki
Institute Of Applied Biochemistry University Of Tsukuba
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MASEDA HIDEAKI
Institute of Applied Biochemistry, University of Tsukuba
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Uchiyama Hiroo
Graduate School Of Life And Environmental Sciences Univ. Of Tsukuba
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Uchiyama H
National Inst. Environmental Studies Ibaraki Jpn
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Uchiyama H
National Institute For Environmental Studies
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Uchiyama Hiroo
National Institute Of Environmental Studies
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Uchiyama Hiroo
Water And Soil Environment Division National Institute For Enviromental Studies
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Nomura Nobuhiko
Graduate School Of Life And Environmental Sciences Univ. Of Tsukuba
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Nomura Nobuhiko
Graduate School Of Life And Environmental Sciences University Of Tsukuba
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Nomura Nobuhiko
Institute Of Applied Biochemistry University Of Tsukuba
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Nakahara Tadaatsu
Institute of Applied Biochemistry, University of Tsukuba
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Maseda H
Institute Of Life And Environmental Sciences University Of Tsukuba
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Maseda Hideaki
Institute Of Applied Biochemistry University Of Tsukuba
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MATSUZAKA EMIKO
Institute ofApplied Biochemistry, University of Tsukuba
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OTAGAKI HIROSHI
Institute ofApplied Biochemistry, University of Tsukuba
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Matsuzaka Emiko
Institute Ofapplied Biochemistry University Of Tsukuba
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Otagaki Hiroshi
Institute Ofapplied Biochemistry University Of Tsukuba
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Nakajima-kambe Toshiaki
Graduate School Of Life And Environmental Sciences Univ. Of Tsukuba
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Nakahara Tadaatsu
Graduate School Of Life And Environmental Science University Of Tsukuba
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Nakahara Tadaatsu
Institute Of Applied Biochemistry University Of Tsukuba
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Nomura Nobuhiko
Institute Of Life And Environmental Sciences University Of Tsukuba
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Uchiyama Hiroo
Institute Of Life And Environmental Sciences University Of Tsukuba
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Nakae T
Graduate School Of Life And Environmental Sciences Univ. Of Tsukuba
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Nomura N
Institute Of Applied Biochemistry University Of Tsukuba
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Nakahara Tadaatsu
Institute Of Applied Biochemistry The University Of Tsukuba
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UCHIYAMA Hiroo
Institute of Applied Biochemistry, University of Tsukuba
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UCHIYAMA Hiroo
Institute of Applied Biochemistry, The University of Tsukuba
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