Trichloroethylene Degradation by Cells of a Methane-Utilizing Bacterium, Methylocystis sp. M, Immobilized in Calcium Alginate
スポンサーリンク
概要
- 論文の詳細を見る
When Methylocystis sp. M cells were immobilized in calcium alginate, the resulting cell beads showed optimum trichloroethylene (TCE) degradation activity at pH 7.0 and 35℃. In comparison with free cells, the immobilized cells were more stable at low pH, and to some extent, at higher temperatures. Studies on the kinetics and the influence of cell density suggest that oxygen permeation was a rate-limiting step. Investigation of the storage stability and the optimum concentration of dissolved oxygen revealed that the TCE degradability was greater under anaerobic than aerobic conditions. Although a toxic effect caused by TCE was observed, methane seemed to restore activity, suggesting that the development of a two-step reactor system might be advantageous. The finding that the immobilized cells showed TCE degradation activity in actual groundwater suggests that TCE bioremediation could be achieved through the use of bioreactors with such cells.
- 社団法人日本生物工学会の論文
- 1995-06-25
著者
-
Uchiyama H
National Inst. Environmental Studies Ibaraki Jpn
-
Uchiyama Hiroo
National Institute Of Environmental Studies
-
Uchiyama Hiroo
National Institute For Environmental Studies
-
Uchiyama Hiroo
Water And Soil Environment Division National Institute For Enviromental Studies
-
YAGI OSAMI
National Institute of Environmental Studies
-
KOKUFUTA ETSUO
Institute of Applied Biochemistry, University of Tsukuba
-
OGURI KAZUHITO
Institute of Applied Biochemistry, University of Tsukuba
-
NISHIBAYASHI MARI
Institute of Applied Biochemistry, University of Tsukuba
-
Yagi Osami
Water And Soil Environmental Studies
-
Yagi Osami
National Institute For Environmental Studies
-
Kokufuta E
Univ. Tsukuba Ibaraki Jpn
-
Kokufuta Etsuo
Institute Of Applied Biochemistry University Of Tsukuba
-
Oguri Kazuhito
Institute Of Applied Biochemistry University Of Tsukuba
-
Yagi O
Univ. Tokyo Tokyo Jpn
-
Nishibayashi Mari
Institute Of Applied Biochemistry University Of Tsukuba
関連論文
- Bacterial growth monitoring in a microfluidic device by confocal reflection microscopy(METHODS)
- Visualizing the Effects of Biofilm Structures on the Influx of Fluorescent Material Using Combined Confocal Reflection and Fluorescent Microscopy
- Monitoring biofilm development in a microfluidic device using modified confocal reflection microscopy(METHODS)
- Rapid monomerization of poly(butylene succinate)-co-(butylene adipate) by Leptothrix sp.(ENVIRONMENTAL BIOTECHNOLOGY)
- Matrix Metalloproteinase-2 Inhibition and Zn^-Chelating Activities of Pyoverdine-Type Siderophores (MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Participation of Nitrite Reductase in Conversion of NO_2^- to NO_3^- in a Heterotrophic Nitrifier, Burkholderia cepacia NH-17, with Denitrification Activity
- Cloning and Sequence Analysis of Poly(tetramethylene succinate) Depolymerase from Acidovorax delafieldii Strain BS-3
- The Response of Pseudomonas aeruginosa PAO1 Efflux Pump-Defective Mutants to N-Octanoyl-L-Homoserine Lactone
- PB-95 A novel method to nondestructively monitor structure development of living biofilms and gaseous metabolic product in anaerobic condition(Additional posters,Poster presentation B)
- Recovery of ^Cs by a Bioaccumulation System Using Rhodococcus erythropolis CS98
- Kinetic Studies on Cesium Transport in Rhodococcus erythropolis CS98 and Rhodococcus sp. Strain CS402
- Trichloroethylene Degradation by Cells of a Methane-Utilizing Bacterium, Methylocystis sp. M, Immobilized in Calcium Alginate
- Identification and Functional Analysis of Genes Required for Desulfurization of Alkyl Dibenzothiophenes of Mycobacterium sp. G3(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Characterization of the Aerobic Denitrification in Mesorhizobium sp. Strain NH-14 in Comparison with that in Related Rhizobia
- Screening of Novel Cellulose-Degrading Bacterium and Its Application to Denitrification of Groundwater(Environmental Biotechnology)
- A Quorum-Sensing Autoinducer Enhances the mexAB-oprM Efflux-Pump Expression without the MexR-Mediated Regulation in Pseudomonas aeruginosa
- Oxidation of Some Alkanes and Trichloroethylene by H_2O_2/Hydroxylase System of Soluble Methane Monooxygenase from Methylocystis sp.M
- Purification and Characterization of Component B of a Soluble Methane Monooxygenase from Methylocystis sp. M
- Sexual Differences in Gonad Fatty Acid Compositions in Dominant Limpets Species from the Sanriku Coast in Northern Japan
- Fatty Acid Compositions of Muscle and Viscera Lipids in Dominant Limpets Species from Otsuchi Bay in Northern Japan
- Purification and Properties of a Soluble Methane Monooxygenase from Methylocystis sp. M
- Novel Metabolite of Trichloroethylene in a Methanotrophic Bacterium, Methylocystis sp. M, and Hypothetical Degradation Pathway
- Protein Phosphatase Inhivition Assay for Detection of Microcystins in Lake Water and Microcystis Cultures
- Random Amplified Polymorphic DNA (RAPD) Analyses for Discriminating Genotypes of Microcystis Cyanobacteria
- Growth Inhibition of Microcystis Cyanobacteria by L-Lysine and Disappearance of Natural Microcystis Blooms with Spraying
- Characteristics of 2-Methylisocitrate Dehydratase, Isolated from Yallowia lipolytica, in Comparison with Aconitase
- Isolation of 2-Methylisocitrate Dehydratase, a New Enzyme Serving in the Methylcitric Acid Cycle for Propionate Metabolism, from Yallowia lipolytica
- Aerobic Degradation of Trichloroethylene by a New Type II Methane-Utilizing Bacterium, Strain M(Microbiology & Fermentation Industry)
- Aerobic Degradation of Trichloroethylene at High Concentration by a Methane-Utilizing Mixed Culture(Microbiology & Fermentation Industry)
- Development of a Most-probable-number Method for Enumerating Denitrifying Bacteria by Using 96-Well Microtiter Plates and an Anaerobic Culture System
- Development of a New Most-probable-number Method for Enumerating Methanotrophs, Using 48-well Microtiter Plates
- Characteristics of Escherichia coli HB101 and Pseudomonas putida PpY101 Harboring a Recombinant Plasmid with Tandem Insertion of the Mercury Resistance Operon
- Selective Determination of Selenite and Selenate in Wastewater by Graphite Furnace AAS after Iron(III) Hydroxide Coprecipitation and Reductive Coprecipitation on Palladium Collector Using Hydrazinium Sulfate
- Coimmobilization of Nitrosomonas europaea and Paracoccus denitrificans Cells Using Polyelectrolyte Complex-Stabilized Calcium Alginate Gel
- Immobilization of Trichloroethylene-Degrading Bacterium, Methylocystis sp. Strain M in Different Matrices
- Continuous Column Denitrfication Using Polyelectrolyte Complex-Entrapped Paracoccus denitrificans Cells
- Immobilization of Paracoccus denitrificans Cells with Polyelectrolyte Complex and Denitrifying Activity of the Immobilized Cells
- New intelligent polymer gels: a self-oscillating gel with pacemaking and actuating functions
- Isolation and Characterization of the Slime from a Cyanobacterium, Microcystis aeruginosa K-3A(Microbiology & Fermentation Industry)
- Degradation Pathways of Trichloroethylene and 1,1,1-Trichloroethane by Mycobacterium sp. TA27(Microbiology & Fermentation Technology)
- Quantitative and Specific Detection of a Trichloroethylene-degrading Methanotroph, Methylocystis sp. Strain M, by a Most Probable Number-Polymerase Chain Reaction Method(Microbiology & Fermentation Technology)
- Quantitative Detection of Trichloroethylene-Degrading Mycobacterium sp. TA27 with a Real-time PCR Product Detection System
- Transformation of Pseudomonas putida by Electroporation
- Survival and Impact of Genetically Engineered Pseudomonas putida Harboring Mercury Resistance Gene in Soil Microcosms
- Survival and Impact of Genetically Engineered Pseudomonas putida Harboring Mercury Resistance Gene in Aquatic Microcosms
- Role of Methylcitric Acid Cycle in Catabolism of Amino Acids by Saccharomycopsis lipolytica(Microbiology & Fermentation Industry)
- Selective Determination of Selenium(IV) and Selenium(VI) in Waste Water by Graphite Furnace AAS after Reductive Coprecipitation on Tellurium Collector by Ascorbic Acid, Tin(II) Chloride and Hydrazinium Sulfate
- Distribution of the Methylcitric Acid Cycle and β-Oxidation Pathway for Propionate Catabolism in Fungi