Characteristics of Escherichia coli HB101 and Pseudomonas putida PpY101 Harboring a Recombinant Plasmid with Tandem Insertion of the Mercury Resistance Operon

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概要

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• We constructed the plasmid pSUPmer2 by inserting tandem copies of the mercury resistance (mer) operon into a broad host range-vector, and introduced it into Escherichia coli HB101 and Pseudomonas putida PpY101 to increase their mercury resistance. Strains harboring plasmid pSUPmer2 had higher mercury resistance and mercuric reductase activity than those strains harboring the plasmid pSUPmer which had one copy of the mer operon. Mercury resistance of P.putida PpY101 was signiticantly increased by tandem insertion of the mer operon.

• 社団法人日本農芸化学会の論文
• 1997-07-23

著者

• UCHIYAMA HIROO

  Institute of Life and Environmental Sciences, University of Tsukuba

• Uchiyama Hiroo

  Graduate School Of Life And Environmental Sciences Univ. Of Tsukuba

• Uchiyama H

  National Inst. Environmental Studies Ibaraki Jpn

• Uchiyama H

  National Institute For Environmental Studies

• Uchiyama Hiroo

  National Institute Of Environmental Studies

• Uchiyama Hiroo

  National Institute For Environmental Studies

• Uchiyama Hiroo

  Water And Soil Environment Division National Institute For Enviromental Studies

• Tanaka Hideo

  Institute Of Life Science And Bioengineering Graduate School Of Life And Environmental Science Unive

• YAGI OSAMI

  National Institute of Environmental Studies

• Yagi Osami

  Water And Soil Environmental Studies

• Yagi Osami

  National Institute For Environmental Studies

• Tanaka H

  Institute Of Applied Biochemistry University Of Tsukuba

• IWASAKI KAZUHIRO

  National Institute for Environmental Studies

• Iwasaki K

  Laboratory Of Biochemistry Department Of Bioscience And Biotechnology Faculty Of Agriculture Graduat

• KURABAYASHI Teruyo

  Institute of Applied Biochemistry, University of Tsukuba

• NAKAMURA Kunihiko

  National Research Center for Minamata Disease

• Yagi O

  Univ. Tokyo Tokyo Jpn

• Nakamura Kunihiko

  National Institute For Minamata Disease

• Iwasaki K

  National Inst. For Environmental Studies Ibaraki Jpn

• Kikuchi T

  Ibaraki Univ. Ibaraki‐ken Jpn

• Tanaka Hideo

  Institute For Biological Resources And Functions National Institute Of Advanced Industrial Science A

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