Purification and Properties of a Soluble Methane Monooxygenase from Methylocystis sp. M
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概要
- 論文の詳細を見る
A soluble methane monooxygenase (sMMO : EC 1.14.13.25) was purified from a type II obligate methanotroph, Methylocystis sp. M. Ion exchange chromatography elution separated the sMMO into three components, I, II, and III. Components II and III were purified to homogeneity and were essential for the sMMO activity. Components II and III had molecular masses of approximately 233,000 and 39,000,respectively. Component II consisted of three subunits with molecular masses of 55,000,44,000,and 21,000,which appeared to be present in stoichiometric amounts, suggesting a (αβγ)_2 configuration in the native protein. Component II contained l-4 mol of iron and was considered to be a hydroxylase. Component III was a flavoprotein, which contained 1 mol of FAD as well as 1-2 mol of iron. It catalyzed the reduction of K_3Fe(CN)_6 and 2,6-dichloroindophenol by NADH. Component I, which was partially purified and not essential for sMMO activity, stimulated the activity by about 11-fold. Its stimulation could be replaced by addition of Fe^<2+>. The molecular mass of the partially purified component I was estimated to be from 35,000 to 40,000 based on gel filtration, which suggested the presence of a new type of regulatory protein of sMMO.
- 社団法人日本農芸化学会の論文
- 1992-05-23
著者
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Nakajima Toshiaki
Institute Of Applied Biochemistry University Of Tsukuba
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NAKAJIMA Terumi
Suntory Institute for Bioorganic Research
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Uchiyama H
National Inst. Environmental Studies Ibaraki Jpn
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Uchiyama Hiroo
National Institute Of Environmental Studies
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Uchiyama Hiroo
National Institute For Environmental Studies
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Uchiyama Hiroo
Water And Soil Environment Division National Institute For Enviromental Studies
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YAGI OSAMI
National Institute of Environmental Studies
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Nakajima T
Suntory Institute For Bioorganic Research
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Nakahara T
Institute Of Applied Biochemistry University Of Tsukuba:(present Address)ryutsu Keizai University
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Yagi Osami
Water And Soil Environmental Studies
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Yagi Osami
National Institute For Environmental Studies
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NAKAHARA Tadaatu
Institute of Applied Biochemistry, University of Tsukuba
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Nakahara Tadaatu
Institute Of Applied Biochemistry University Of Tsukuba
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Yagi O
Univ. Tokyo Tokyo Jpn
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