Protein Phosphatase Inhivition Assay for Detection of Microcystins in Lake Water and Microcystis Cultures

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概要

• 論文の詳細を見る

• The protein phosphatase inhibition assay was used to quantify low levels of microcystin in lake water polluted by cyanobacterial blooms of Microcystis, and toxin concentrations in cultures of 15 strains of Microcystis. This method was highly sensitive permitting measurement of microcystin in concentrations of the order of μg1^<-1>. The concentrations of microcystin in the surface water of Lake Kasumigaura in the summer of 1995 were in the range of 0.16 to 2.7μg1^<-1> microcystin-LR equivalents. On the other hand, markedly high levels of microcystin (160 to 1,429μg1^<-1>) were measured in the culture fluids of M. aeruginosa TAC 192-2,M. ichthyoblabe TAC 69,M. ichthyoblabe TAC 113-1,M. viridis TAC 45-1,M. viridis TAC 64 and M. viridis TAC 92. There were strains which did not produce microcystin in the culture fluid within the same species of Microcystis. Therefore, the toxin productivity in the culture fluid is not a useful criterion for discrimination of the species of Microcystis.

• 日本微生物生態学会の論文
• 1998-09-30

著者

• WATANABE Masayuki

  Department of Pediatrics and Developmental Medicine, Gunma University Graduate School of Medicine

• Yagi Osami

  Water and Soil Environment Division, National Institute for Environmental Studies

• Watanabe Masayuki

  Department Of Botany National Science Museum

• Yagi Osami

  Water And Soil Environmental Studies

• YAO YASUKO

  Applied Technology Research Center, NKK Corporation

• UJIIE YUMI

  Analytical Center, Kokan Keisoku Service

• TAKAMURA YOSHICHIKA

  Department of Bioresource Science, Faculty of Agriculture, Ibaraki University

• Ujiie Yumi

  Analytical Center Kokan Keisoku Service

• Yao Yasuko

  Applied Technology Research Center Nkk Corporation

• Yagi O

  Univ. Tokyo Tokyo Jpn

• Takamura Y

  Department Of Bioresources Sciences School Of Agriculture Ibaraki University

• Takamura Yoshichika

  Department Of Agricultural Chemistry Faculty Of Agriculture Ibaraki University

• Watanabe Masayuki

  Department Of Biology Graduate School Of Natural Science Konan University

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