Characteristics of 2-Methylisocitrate Dehydratase, Isolated from Yallowia lipolytica, in Comparison with Aconitase
スポンサーリンク
概要
- 論文の詳細を見る
A new enzyme, 2-methylisocitrate dehydratase, isolated from Yallowia lipolytica, functioning in the methylcitric acid cycle for propionate metabolism, had a pI of 4.4 and a M_r of 69,500. The enzyme was composed of 624 residues of amino acids per molecule. No cofactor was required for full enzyme activity. The enzyme was competitively inhibited by threo-D_s-isocitrate (K_i=68mM), but not by any other tested metabolites. The enzyme was weakly inhibited by some thiol reagents, but not by any metal-chelating reagents, differing from aconitase, which dehydrates 2-methylisocitrate. This difference between the enzymes made it possible to estimate the activity of the new enzyme even in crude cell-free extracts. The enzyme was constitutively synthesized, but had no regulatory function in the methylcitric acid cycle. The enzyme was supposed to have evolutionarily developed from a hypothetical and prototypical isocitrate dehydratase.
- 社団法人日本農芸化学会の論文
- 1995-11-23
著者
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Aoki H
Iwate Univ. Iwate Jpn
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Aoki Hideyuki
Research Laboratory Ikeda Food Research Co. Ltd.
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Aoki H
Ikeda Food Res. Co. Ltd. Hiroshima Jpn
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Uchiyama Hiroo
Graduate School Of Life And Environmental Sciences Univ. Of Tsukuba
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Uchiyama H
National Inst. Environmental Studies Ibaraki Jpn
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Uchiyama H
National Institute For Environmental Studies
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Uchiyama Hiroo
National Institute Of Environmental Studies
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Uchiyama Hiroo
Water And Soil Environment Division National Institute For Enviromental Studies
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TABUCHI Takeshi
Institute of Applied Biochemistry, University of Tsukuba
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Tabuchi T
Institute Of Bioscience And Biotechnology Aomori University
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Tabuchi Takeshi
Institute Of Applied Biochemistry The University Of Tsukuba
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Umetsu H
Japan Women's Univ. Tokyo Jpn
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Umetsu Hironori
Institute Of Bioscience And Biotechnology Aomori University
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Aoki Hiroyuki
Institute of Applied Biochemistry, University of Tsukuba
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Uchiyama Hirr
National Institute for Environmental Studies
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Aoki Hiroyuki
Institute of Applied Biochemistry, The University of Tsukuba
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