Construction of an Efficient Expression System for Aspergillus Isopullulanase in Pichia pastoris, and a Simple Purification Method(Biochemistry & Molecular Biology)

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概要

• 論文の詳細を見る

• Aspergillus niger ATCC 9642 isopullulanase (IPU) was heterologously expressed by Pichia pastoris GS115 under three different signal sequences of Saccharomyces cerevisiae acid phosphatase, S. cerevisiae α-factor prepro peptide, and A. niger isopullulanase. One-step purification using lectin Con A affinity chromatography yielded recombinant IPU (IPU-PP) with high purity. IPU-PP had a higher carbohydrate content than native IPU and IPU-AO expressed in A. oryzae M-2-3. IPU-PP hydrolyzed various substrates containing the structure of panose, which indicated a strict subsite recognition of the panose motif.

• 社団法人日本農芸化学会の論文
• 2003-05-23

著者

• Aoki H

  Iwate Univ. Iwate Jpn

• Aoki Hideyuki

  Research Laboratory Ikeda Food Research Co. Ltd.

• NISHIKAWA ATSUSHI

  Department of Mechanical Science and Bioengineering, Graduate School of Engineering Science, Osaka U

• Kashiwagi Yutaka

  National Food Research Institute

• Aoki H

  Ikeda Food Res. Co. Ltd. Hiroshima Jpn

• TONOZUKA Takashi

  Department of Applied Biological Science, Faculty of Agriculture, Tokyo University of Agriculture an

• 殿塚 隆史

  東京農工大学農学部

• SAKANO Yoshiyuki

  Department of Applied Biological Science, Tokyo University of Agriculture Technology

• AKEBOSHI Hiromi

  Department of Applied Biological Science, Faculty of Agriculture, Tokyo University of Agriculture an

• AOKI Hiroyoshi

  Department of Applied Biological Science, Faculty of Agriculture, Tokyo University of Agriculture an

• Aoki Hiroyoshi

  Department Of Applied Biological Science Faculty Of Agriculture Tokyo University Of Agriculture And

• Tonozuka Takashi

  Department Of Applied Biological Science Faculty Of Agriculture Tokyo University Of Agriculture And

• Nishikawa A

  Department Of Applied Biological Science Faculty Of Agriculture Tokyo University Of Agriculture And

• Nishikawa Atsushi

  Department Of Mechanical Science And Bioengineering Graduate School Of Engineering Science Osaka Uni

• Sakano Y

  Department Of Applied Biological Science Faculty Of Agriculture Tokyo University Of Agriculture And

• Sakano Yoshiyuki

  Department Of Applied Biological Science Faculty Of Agriculture Tokyo University Of Agriculture And

• Nishikawa Atsushi

  Department Of Applied Biological Science Tokyo University Of Agriculture And Technology

• Tonozuka Takashi

  Forage Crop Breeding Unit National Agricultural Research Center For Kyushu Okinawa Region

• SAKANO Yoshiyuki

  Department of Agricultural Chemistry, Faculty of Agriculture, Tokyo Noko University

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