The Nucleotide Sequence of the β-Glucosidase Gene from Cellvibrio gilvus
スポンサーリンク
概要
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A gene encoding β-glucosidase from Cellvibrio gilvus, a cellobiose-producing bacterium, was cloned into Escherichia coli and sequenced. The structural gene consisted of 2565 bp encoding 854 amino acid residues with a characteristic signal peptide. A typical promoter sequence and SD region were located upstream of the initiation ATG codon. A sequence (180 amino acids) having high homology with those of β-glucosidases from several microorganisms was found in the deduced amino acid sequence of C. gilvus β-glucosidase. This sequence contains the aspartic acid residue which was found to be an active site residue in Aspergillus wentii β-glucosidase A3. The β-glucosidase gene of C. gilvus contains a high amount (69.4%) of G+C. These bases are localized not in the 3rd position of the codon, as is usually observed in G+C-rich genes, but rather in the 1st position. This result in a peptide which contains an extremely high amount (48%) of four amino acids (Pro, Ala, Arg, Gly) coded by CCN, GCN, CGN, and GGN.
- 公益社団法人日本生物工学会の論文
- 1993-03-25
著者
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Sawa T
Institute Of Microbial Chemistry
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Kashiwagi Yutaka
National Food Research Institute
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Sasaki Takashi
National Food Research Institute
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Taniguchi Hajime
National Food Research Institute
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AOYAGI CHIKA
National Food Research Institute, Kannondai
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Aoyagi C
National Food Res. Inst. Ibaraki Jpn
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Aoyagi Chika
National Food Research Institute Kannondai
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Taniguchi H
Yamaguchi Univ. Yamaguchi Jpn
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Kashiwagi Y
National Food Research Institute
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Taniguchi Hajime
National Food Research Insitute, Ministry of Agricultuer, Forestry and Fisheries
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