Cloned Cytosine Deaminase Gene Expression of Bifidobacterium longum and Application to Enzyme/Pro-drug Therapy of Hypoxic Solid Tumors(Biochemistry & Molecular Biology)
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概要
- 論文の詳細を見る
Bifidobacterium longum is a nonpathogenic anaerobic bacterium among normal bacterial flora. Recently, it was reported that B. longum accumulated in hypoxic solid tumors. The gene of interest was expressed in transfected B. longum by the shuttle vector pBLES100 in solid tumors. In this report, we constructed pBLES100-S-eCD, which included the cytosine deaminase gene. We confirmed by western blotting that transfected B. longum produced cytosine deaminase. In addition, transfected B. longum produced cytosine deaminase that converted 5-fluorocytosine into 5-fluorouracil. B. longum could be useful for enzyme/pro-drug therapy of hypoxic solid tumors.
- 社団法人日本農芸化学会の論文
- 2002-11-23
著者
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Kano Yasunobu
Department of Molecular Genetics, Kyoto Pharmaceutical University
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Sawa T
Institute Of Microbial Chemistry
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AMANO JUN
Department of Cardiovascular Surgery, Tokyo Medical and Dental University
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Amano J
信州大学 医学部外科
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NAKAMURA Toshiyuki
Department of Internal Medicine, Gifu Red Cross Hospital
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FUJIMORI Minoru
Department of Surgery, Shinshu University School of Medicine
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Taniguchi Shun'ichiro
Dep. Of Molecular Oncology Inst. On Aging And Adaptation Shinshu Univ. Graduate School Of Medicine
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Amano Jun
Department Of Cardio-thoracic Surgery Juntendo Univ. School Of Med. Tokyo Med.& Dent- Univ.
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SASAKI Takayuki
Department of Surgery, Shinshu University School of Medicine
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YAZAWA Kazuyuki
Department of Surgery, Shinshu University School of Medicine
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Kano Y
Kyoto Pharmaceutical Univ. Kyoto Jpn
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Kano Yasunobu
Department Of Molecular Genetics Institute Of Molecular And Cellular Biology For Pharmaceutical Scie
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Fujimori Minoru
Department Of Breast Surgery Tokyo Medical University Ibaraki Medical Center
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Fujimori Minoru
Department Of Surgery Shinshu University School Of Medicine
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Yazawa Kazuyuki
Department Of Surgery Shinshu University School Of Medicine
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Amano J
Dep. Of Surgery Shinshu Univ. School Of Medicine
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Yazawa Kazuyuki
Department Of Electrical Engineering Tokyo Metropolitan University
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Taniguchi Shin-ichi
Department Of Molecular Oncology Institute On Aging And Adaptation Shinshu University Graduate Schoo
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Fujimori Minoru
Anaeropharma Science Inc.
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Sasaki Takayuki
Department Of Nuclear Engineering Kyoto University
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Nakamura Toshiyuki
Department Of Surgery Shinshu University School Of Medicine
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Nakamura Toshiyuki
Department Of Internal Medicine Gifu Red Cross Hospital
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Nakamura Toshiyuki
Department Of Biology Faculty Of Science Kobe University
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Sasaki Takayuki
Department Of Biology Faculty Of Science Niigata University
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Sasaki Takayuki
Institute Of Plant Science And Resources Okayama University
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