Construction of Escherichia coli-Bifidobacterium longum Shuttle Vector Transforming B. longum 105-A and 108-A
スポンサーリンク
概要
- 論文の詳細を見る
A shuttle vector, pBLES100, was constructed by cloning a Bifidobacterium longum plasmid and a gene encoding spectinomycin adenyltransferase AAD(9) from Enterococcus faecalis into the Escherichia coli vector pBR322. Stable transformants with this plasmid were obtained with an efficiency of 2.2×10^4 transformants/μg DNA or 6.9×10^<-5> transformants/cell/μg DNA under the optimal conditions of 10.0 kV/cm, 200Ω, and 25μF, using B. longum 105-A harvested at late log phase of growth.
- 社団法人日本農芸化学会の論文
- 1997-07-23
著者
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Kano Yasunobu
Department of Molecular Genetics, Kyoto Pharmaceutical University
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Kano Y
Kyoto Pharmaceutical Univ. Kyoto Jpn
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Kano Yasunobu
Department Of Molecular Genetics Institute Of Molecular And Cellular Biology For Pharmaceutical Scie
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Matsumura H
Department Of Molecular Genetics Institute Of Molecular And Cellular Biology For Pharmaceutical Scie
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TAKEUCHI Akio
Department of Molecular Genetics, Institute of Molecular and Cellular Biology for Pharmaceutical Sci
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MATSUMURA Hajime
Department of Molecular Genetics, Institute of Molecular and Cellular Biology for Pharmaceutical Sci
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Takeuchi Asako
Department Of Molecular Genetics Institute Of Molecular And Cellular Biology For Pharmaceutical Scie
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Matsumura H
Department Of Molecular Genetics Institute Of Molecular And Cellular Biology For Pharmaceutical Sciences Kyoto Pharmaceutical University
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