Structural and Functional Analysis of pTB6 from Bifidobacterium longum
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概要
- 論文の詳細を見る
The complete nucleotide sequence for pTB6 (3,624 bp) from Bifidobacterium longum was determined. This plasmid is 95% homologous in nucleotide (nuc) sequence, and also 92% in RepB aa sequence, to rolling circle replication (RCR) plasmids pKJ36 and pB44, suggesting that pTB6 replicates by the rolling circle mechanism. The putative MembB, MobA, and protein encoding from orf (Orf) I detected were nonessential for plasmid replication. We constructed an immobile shuttle vector from pTB6 and pUC18, which transformed B. longum with a high efficiency of 2.5×106 transformants/μg DNA.
- 社団法人 日本農芸化学会の論文
- 2005-02-23
著者
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Kano Yasunobu
Department Of Molecular Genetics Institute Of Molecular And Cellular Biology For Pharmaceutical Scie
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TANAKA Kunihito
Department of Molecular Genetics, Institute of Molecular and Cellular Biology for Pharmaceutical Sci
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SAMURA Kazuya
Department of Molecular Genetics, Institute of Molecular and Cellular Biology for Pharmaceutical Sci
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Tanaka K
Department Of Molecular Genetics Institute Of Molecular And Cellular Biology For Pharmaceutical Scie
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Tanaka Kunihito
Department Of Chemistry Faculty Of Science And Technology Sophia University
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Samura Kazuya
Department Of Molecular Genetics Institute Of Molecular And Cellular Biology For Pharmaceutical Scie
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