Strong Enhancement of Recombinant Cytosine Deaminase Activity in Bifidobacterium longum for Tumor-Targeting Enzyme/Prodrug Therapy
スポンサーリンク
概要
- 論文の詳細を見る
In our previous studies, a strain of the nonpathogenic, anaerobic, intestinal bacterium, Bifidobacterium longum (B. longum), was found to be localized selectively and to proliferate within solid tumors after systemic administration. In addition, B. longum transformed with the shuttle-plasmid encoding the cytosine deaminase (CD) gene expressed active CD, which deaminated the prodrug 5-fluorocytosine (5-FC) to the anticancer agent 5-fluorouracil (5-FU). We also reported antitumor efficacy with the same plasmid in several animal experiments. In this study, we constructed a novel shuttle-plasmid, pAV001-HU-eCD-M968, which included the mutant CD gene with a mutation at the active site to increase the enzymatic activity.In addition, the plasmid-transformed B. longum produces mutant CD and strongly increased (by 10-fold) its 5-FC to 5-FU enzymatic activity. The use of B. longum harboring the new shuttle-plasmid increases the effectiveness of our enzyme/prodrug strategy.
- 社団法人 日本農芸化学会の論文
- 2007-04-23
著者
-
Kano Yasunobu
Department of Molecular Genetics, Kyoto Pharmaceutical University
-
Takahashi S
Cancer Prevention Basic Research Project National Cancer Center Research Institute
-
Sawa T
Institute Of Microbial Chemistry
-
AMANO JUN
Department of Cardiovascular Surgery, Tokyo Medical and Dental University
-
Amano J
信州大学 医学部外科
-
FUJIMORI Minoru
Department of Surgery, Shinshu University School of Medicine
-
Taniguchi Shun'ichiro
Dep. Of Molecular Oncology Inst. On Aging And Adaptation Shinshu Univ. Graduate School Of Medicine
-
Amano Jun
Department Of Cardio-thoracic Surgery Juntendo Univ. School Of Med. Tokyo Med.& Dent- Univ.
-
SASAKI Takayuki
Department of Surgery, Shinshu University School of Medicine
-
HAMAJI Yoshinori
Department of Surgery, Shinshu University School of Medicine
-
Hama Yoshihisa
Department Of Surgery Shinshu University School Of Medicine
-
Kano Y
Kyoto Pharmaceutical Univ. Kyoto Jpn
-
Kano Yasunobu
Department Of Molecular Genetics Institute Of Molecular And Cellular Biology For Pharmaceutical Scie
-
Shimokawa Takashi
Natl. Inst. Radiol. Sci.
-
Fujimori Minoru
Department Of Breast Surgery Tokyo Medical University Ibaraki Medical Center
-
Fujimori Minoru
Department Of Surgery Shinshu University School Of Medicine
-
Amano J
Dep. Of Surgery Shinshu Univ. School Of Medicine
-
Taniguchi Shin-ichi
Department Of Molecular Oncology Institute On Aging And Adaptation Shinshu University Graduate Schoo
-
Shimatani Shibata
Department Of Surgery Shinshu University School Of Medicine
-
Fujimori Minoru
Anaeropharma Science Inc.
-
MATSUHASHI Hitomi
Department of Surgery, Shinshu University School of Medicine
-
MATSUI SEKI
Department of Surgery, Shinshu University School of Medicine
-
Tamai Susumu
Cancer Prevention Division National Cancer Center Research Institute
-
Sasaki Takayuki
Department Of Nuclear Engineering Kyoto University
-
Sugimura Takashi
Carcinogenesis Division National Cancer Center Research Institute
-
Matsui Seki
Department Of Surgery Shinshu University School Of Medicine
-
Hamaji Yoshinori
Department Of Surgery Shinshu University School Of Medicine
-
Matsuhashi Hitomi
Department Of Surgery Shinshu University School Of Medicine
-
Sasaki Takayuki
Department Of Biology Faculty Of Science Niigata University
-
Sasaki Takayuki
Institute Of Plant Science And Resources Okayama University
関連論文
- Characterization of an insertion sequence-like element, ISBlo15, identified in a size-increased cryptic plasmid pBK283 in Bifidobacterium longum BK28(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Helicobacter pylori Infection, Serum Pepsinogen Level and Gastric Cancer : A Case-Control Study in Japan
- Frequent hypomethylation in multiple promoter CpG islands is associated with global hypomethylation, but not with frequent promoter hypermethylation
- Cloning of the 5' Upstream Region of the Rat p16 Gene and Its Role in Silencing
- Inhibition of cell proliferation by nobiletin, a dietary phytochemical, associated with apoptosis and characteristic gene expression, but lack of effect on early rat hepatocarcinogenesis in vivo
- Periodic analysis of urethane-induced pulmonary tumors in living A/J mice by respiration-gated X-ray microcomputed tomography
- Immunocytochemical Analysis of the AtheroscLerotic lesion : 54th Annual Scientific Session of the Japanese Circulation Society
- Induction of multinucleated cells and apoptosis in the PC-3 prostate cancer cell line by low concentrations of polyethylene glycol 1000
- Mouse strain differences in inflammatory responses of colonic mucosa induced by dextran sulfate sodium cause differential susceptibility to PhIP-induced large bowel carcinogenesis
- Toxicogenomic approach to the prediction of carcinogenic potentials of heterocyclic amines in rat colon(Analysis of Toxicological Mechanisms : A Toxicogenomics Approach, Proceedings of the 32nd Annual Meeting)
- HETEROCYCLIC AMINES : MUTAGENS/CARCINOGENS PRODUCED DURING COOKING OF MEAT AND FISH
- Heterocyclic amines : Mutagens/carcinogens produced during cooking of meat and fish
- Differential gene expression profiles in colon epithelium of two rat strains with distinct susceptibility to colon carcinogenesis after exposure to PhlP in combination with dietary high fat
- Parp-1 deficiency implicated in colon and liver tumorigenesis induced by azoxymethane
- Induction of Intestinal Tumors and Lymphomas in C57BL/6N Mice by a Food-borne Carcinogen, 2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyridine
- Frequent and Multiple Mutations at Minisatellite Loci in Sporadic Human Colorectal and Gastric Cancers : Possible Mechanisble Mechanistic Differences from Microsatellite Instability in Cancer Cells
- Migrastatin, a Novel 14-Membered Lactone from Streptomyces sp. MK929-43F1
- Lactonamycin, a New Antimicrobial Antibiotic Produced by Streptomyces rishiriensis MJ773-88K4 II. Structure Determination
- Lactonamycin, a New Antimicrobial Antibiotic Produced by Streptomyces rishiriensis MJ773-88K4 I. Taxonomy, Fermentation, Isolation, Physico-chemical properties and Biological Activities
- Biosynthesis of Neothramycin
- Kinetic Studies on p-Nitrophenyl-cellobioside Hydrolyzing Xylanase from Cellvibrio gilvus
- Phosphorolytic Reaction of Cellvibrio gilvus Cellobiose Phosphorylase
- Purification and Properties of a Xylanase from Cellvibrio gilvus That Hydrolyzes p-Nitrophenyl Cellooligosaccharides(Biological Chemistry)
- Synthesis of Laminarioligosaccharides Using Crude Extract of Euglena gracilis z Cells(Biological Chemistry)
- A Simple Method of Cellulase Immobilization on a Modified Silica Support
- 5 Autologous Mononuclear Cell Implantation Therapy for Critical Digit Ischemia in Patients with Connective Tissue Diseases(Regenerative Therapy for Peripheral Artery Disease,Symposium 12 (SY-12) (I),The 73rd Annual Scientific Meeting of The Japanese Circu
- Safety Evaluation of Flavonoids-Containing Tomato Skin Extract
- Occurrence of mutations in the epidermal growth factor receptor gene in X-ray-induced rat lung tumors
- Phylogenic distribution of poly(ADP-ribose) glycohydrolase and poly(ADP-ribose)-digesting phosphodiesterase
- Isolation and Cloning of Rat Poly (ADP-Ribose) Glycohydrolase: Presence of a Potential Nuclear Export Signal Conserved in Mammalian Orthologs
- Presence in Pieris rapae of Cytotoxic Activity against Human Carcinoma Cells
- PHYSIOLOGYCAL ROLE OF A APOPTOSIS-INDUCING PROTEIN, PIERISIN-1, IN CABBAGE BUTTERFLY, PIERIS RAPAE(Endocrinology,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)
- GERMLINE MUTATIONS OF THE APC GENE IN TWO JAPANESE ADENOMATOUS POLYPOSIS PATIENTS
- PJ-689 Painless Acute Aortic Dissection-Diagnostic, Prognostic, and Clinical Implications(Peripheral circulation/Vascular disease-6, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- Purification of Pierisin, an Inducer of Apoptosis in Human Gastric Carcinoma Cells, from Cabbage Butterfly, Pieris rapae
- Heart failure in the dog induced by rapid ventricular pacing using a newly designed cardiac pacemaker, MCMS-0102
- Detection of endogenous DNA adducts, O^6-carboxymethyl-2'-deoxyguanosine and 3-ethanesulfonic acid-2'-deoxycytidine, in the rat stomach after duodenal reflux
- Enzymatic Properties of Pierisin-1 and Its N-Terminal Domain, a Guanine-Specific ADP-Ribosyltransferase from the Cabbage Butterfly
- OE-137 Pivotal Roles of Inflammatory Factors in Arteriosclerosis and its Specific Regulation(Infection/Inflammation/Immunity-1, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- IS023 Antisense Proliferating-cell Nuclear Antigen Oligonucleotide Attenuates Cardiac Allograft Arteriopathy
- Successful Surgical Treatment for Acute Aortic Dissection in Pregnancy with Marfan's Syndrome
- Update of Surgical Treatment for PAD(An Update on the Contemporary Management of Peripheral Arterial Disease, The 69th Annual Scientific Meeting of the Japanese Circulation Society)
- Detection and Cloning of a Protein Recognized by Anti-human Prostate-specific Antigen (PSA) Antibody in the Rat Ventral Prostate
- Induction of GM-CSF Production of Macrophages by Advanced Glycation End Products of the Maillard Reaction
- The Effects of Interleukin (IL)-4 and IL-10 on Macrophage Growth-Stimulating Activities of Oxidized Low Density Lipoprotein (LDL), Acetylated LDL and Macrophage Colony-Stimulating Factor : The activity of Oxidized LDL Is Refractory to the Inhibitory Cytok
- Stimulation of Macrophage DNA Synthesis by Polyanionic Substances through Binding to the Macrophage Scavenger Receptor
- Granular Cell Tumor of the Mediastinum
- TREATMENT OF LEFT VENTRICULAR FAILURE : COMBINED USE OF PHARMACOLOGICAL AGENTS WITH IABP : PROCEEDINGS OF THE 47th ANNUAL SCIENTIFIC MEETING OF THE JAPANESE CIRCULATION SOCIETY : Heart Failure
- STUDIES ON THE SIGNIFICANCE OF SERUM MITOCHONDRIAL ASPARTATE AMINOTRANSFERASE ACTIVITY FOLLOWING ISCHEMIC CARDIAC ARREST
- RAPID PROGRESSION OF CORONARY ARTERY DISEASE : COMPARISON OF CINEANGIOGRAPHIC AND NECROPSY FINDINGS IN THE SURGICAL PATIENT : Ichemic Heart Disease (I) : 46th Annual Scientific Meeting, Japanese Circulation Society
- THE OPTIMAL FLOW RATE DURING REPERFUSION AFTER HYPOTHERMIC ISCHEMIC ARREST
- OPTIMAL FLOW RATE DURING REPERFUSION AFTER CARDIOPLEGIA : Cardiovascular surgery : FREE COMMUNICATIONS (Abstract) : 45 Annual Scientific Meeting, Japanese Circulation Society
- STUDIES ON THE SIGNIFICANCE OF MITOCHONDRIAL ASPARTATE AMINOTRANSFERASE ACTIVITY FOLLOWING HYPOTHERMIC CARDIOPLEGIA : Ventricular function (I) : FREE COMMUNICATIONS (Abstract) : 45 Annual Scientific Meeting, Japanese Circulation Society
- ADDITIVE PROTECTION OF APROTINN, PROTEASE INHIBITOR TO COLD CARDIOPLEGIA FROM ISCHEMIC MYOCARIUM
- A Subtotal Sternectomy Successfully Reconstructed with Composix Mesh^
- Giant intraatrial septal aneurysm originating from a branch of the left coronary artery
- Migrastatin, a New Inhibitor of Tumor Cell Migration from Streptomyces sp. MK929-43F1 : Taxonomy, Fermentation, Isolation and Biological Activities
- Genetically engineered Bifidobacterium longum for tumor-targeting enzyme-prodrug therapy of autochthonous mammary tumors in rats
- Cardiac Myxoma : Its Origin and Tumor Characteristics
- Cloned Cytosine Deaminase Gene Expression of Bifidobacterium longum and Application to Enzyme/Pro-drug Therapy of Hypoxic Solid Tumors(Biochemistry & Molecular Biology)
- Stromal sarcoma of the breast with leiomyosarcomatous pattern
- The Likely Transformation of Papillary Thyroid Carcinoma into Anaplastic Carcinoma During Postoperative Radioactive Iodine-131 Therapy : Report of a Case
- Inhibition of Rat Embryo Histidine Decarboxylase by Epoxyquinomicins
- MK800-62F1, a New Inhibitor of Apoptotic Cell Death, from Streptomyces diastatochromogenes MK800-62F1 : II. Structure Elucidation
- MK800-62F1, a New Inhibitor of Apoptotic Cell Death, from Streptomyces diastatochromogenes MK800-62F1 : I. Taxonomy, Fermentation, Isolation, Physico-chemical Properties and Biological Activity
- C-1027 Enediyne Chromophore: Presence of Another Active Form and Its Chemical Structure
- Diperamycin, a New Antimicrobial Antibiotic Produced by Streptomyces griseoaurantiacus MK393-AF2 I. Taxonomy, Fermentation, Isolation, Physico-chemical Properties and Biological Activities
- MJ347-81F4 A & B, Novel Antibiotics from Amycolatopsis sp.: Taxonomic Characteristics, Fermentation, and Antimicrobial Activity
- Effect of a Novel Antibiotic, Heliquinomycin, on DNA Helicase and Cell Growth
- Cremimycin, a Novel 19-Membered Macrocyclic Lactam Antibiotic, from Streptomyces sp.
- Epoxyquinomicins A, B, C and D, New Antibiotics from Amycolatopsis III. Physico-chemical Properties and Structure Determination
- Epoxyquinomicins A, B, C and D, New Antibiotics from Amycolatopsis II. Effect on Type II Collagen-induced Arthritis in Mice
- Epoxyquinomicins A, B, C and D, New Antibiotics from Amycolatopsis I. Taxonomy, Fermentation, Isolation and Antimicrobial Activities
- Discovery of Methyl-2-(2'-hydroxyphenyl)-2-oxazoline-4-carboxylate as a Secondary Metabolite from Actinomadura sp.
- Heliquinomycin, a New Inhibitor of DNA Helicase, Produced by Streptomyces sp. MJ929-SF2 III. Biosynthesis
- A New Anthracycline Antibiotic, IT-62-B, Converts the Morphology of ras-Transformed Cells Back to Normal: Taxonomy, Fermentation, Isolation, Structure Elucidation and Biological Characterization
- Pyralomicins, Novel Antibiotics from Microtetraspora spiralis IV. Absolute Configuration
- Heliquinomycin, a New Inhibitor of DNA Helicase, Produced by Streptomyces sp. MJ929-SF2 II. Structure Determination of Heliquinomycin
- Imaging study of pancreatic ductal adenocarcinomas in Syrian hamsters using X-ray micro-computed tomography (CT)
- Suppression of azoxymethane-induced colon cancer development in rats by a cyclooxygenase-1 selective inhibitor, mofezolac
- Inhibition of intestinal carcinogenesis by a new flavone derivative, chafuroside, in oolong tea
- Cloning of Mongolian gerbil cDNAs encoding inflammatory proteins, and their expression in glandular stomach during H. pylori infection
- Dose-dependent suppression of hyperlipidemia and intestinal polyp formation in Min mice by pioglitazone, a PPARγ ligand
- The Absence of Mth1 Inactivation and DNA Polymerase κ Overexpression in Rat Mammary Carcinomas with Frequent A:T to C:G Transversions
- Linkage Mapping of the Rat Interleukin 1 Receptor Antagonist Gene on Chromosome 3
- UV-lnduced Apoptosis in Thymic Lymphoma Cells : Cell Cycle uependency.
- Effects of Serine Protease Inhibitors on the Radiation-induced Apoptosis in Thymocytes and Lymphoma Cells.
- Cloning and Expression in Escherichia coli of a Gene, hup, Encoding the Histone-like Protein HU of Bifidobacterium longum(Microbiology & Fermentation Technology)
- Construction of Escherichia coli-Bifidobacterium longum Shuttle Vector Transforming B. longum 105-A and 108-A
- Closing Plant Stomata Requires a Homolog of an Aluminum-Activated Malate Transporter
- Antihypertensive and Natriuretic Effects of Less-Sodium Soy Sauce Containing γ-Aminobutyric Acid in Spontaneously Hypertensive Rats
- Targeting solid tumors with non-pathogenic obligate anaerobic bacteria
- Exogeneous Cytosine Deaminase Gene Expression in Bifidobacterium breve I-53-8w for Tumor-Targeting Enzyme/Prodrug Therapy
- Strong Enhancement of Recombinant Cytosine Deaminase Activity in Bifidobacterium longum for Tumor-Targeting Enzyme/Prodrug Therapy
- The Nucleotide Sequence of the β-Glucosidase Gene from Cellvibrio gilvus
- The Nucleotide Sequence of the β-Glucosidase Gene from Cellvibrio gilvus
- Characterization of a β-Glucosidase Encoded by a Gene from Cellvibrio gilvus(Biological Chemistry)
- Inhibitory Effects of Methylglyoxal on Light-Induced Stomatal Opening and Inward K^+ Channel Activity in Arabidopsis
- Paleic acid, a fatty acid from Paenibacillus sp. : taxonomy, fermentation, isolation, structure determination, and anti-Mannheimia and-Pasteurella activity
- Technological Advances in Bifidobacterial Molecular Genetics : Application to Functional Genomics and Medical Treatments