Exogeneous Cytosine Deaminase Gene Expression in Bifidobacterium breve I-53-8w for Tumor-Targeting Enzyme/Prodrug Therapy

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概要

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• Bifidobacteria are nonpathogenic, anaerobic domestic bacteria with health-promoting properties for the host. In our previous study, Bifidobacterium longum (B. longum) were found to be localized selectively and to proliferate within solid tumors after systemic application. Additionally, B. longum transformed by shuttle-plasmid including the cytosine deaminase (CD) gene expressed active CD, converted the prodrug 5-fluorocytosine (5-FC) to 5-fluorouracil (5-FU). We also demonstrated antitumor efficacy with a transformant of B. longum in rats. In this study, we found that Bifidobacterium breve (B. breve), the smallest species of human-derived bifidobacterium, expressed the exogenous transgene (CD), that CD enzymatic activity in the transformant of B. breve was much higher, and that the segregational stability of the plasmid was greater than that of B. longum. Thus, numerous transformants of B. breve were detected solely in the tumors after systemic administration. We consider the transformant of B. breve to be more beneficial in our enzyme/prodrug therapy.

• 社団法人 日本農芸化学会の論文
• 2007-12-23

著者

• Takahashi S

  Cancer Prevention Basic Research Project National Cancer Center Research Institute

• Sawa T

  Institute Of Microbial Chemistry

• FUJIMORI Minoru

  Department of Surgery, Shinshu University School of Medicine

• Taniguchi Shun'ichiro

  Dep. Of Molecular Oncology Inst. On Aging And Adaptation Shinshu Univ. Graduate School Of Medicine

• SASAKI Takayuki

  Department of Surgery, Shinshu University School of Medicine

• HAMAJI Yoshinori

  Department of Surgery, Shinshu University School of Medicine

• Hama Yoshihisa

  Department Of Surgery Shinshu University School Of Medicine

• Shimokawa Takashi

  Natl. Inst. Radiol. Sci.

• Fujimori Minoru

  Department Of Breast Surgery Tokyo Medical University Ibaraki Medical Center

• Fujimori Minoru

  Department Of Surgery Shinshu University School Of Medicine

• Taniguchi Shin-ichi

  Department Of Molecular Oncology Institute On Aging And Adaptation Shinshu University Graduate Schoo

• Fujimori Minoru

  Anaeropharma Science Inc.

• HIDAKA Ayumi

  Department of Surgery, Shinshu University School of Medicine

• Tamai Susumu

  Cancer Prevention Division National Cancer Center Research Institute

• Sasaki Takayuki

  Department Of Nuclear Engineering Kyoto University

• Sugimura Takashi

  Carcinogenesis Division National Cancer Center Research Institute

• Hamaji Yoshinori

  Department Of Surgery Shinshu University School Of Medicine

• Sasaki Takayuki

  Department Of Biology Faculty Of Science Niigata University

• Sasaki Takayuki

  Institute Of Plant Science And Resources Okayama University

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