Characterization of the Biosynthetic Gene Cluster of Rebeccamycin from Lechevalieria aerocolonigenes ATCC 39243(Biochemistry & Molecular Biology)

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概要

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• The biosynthetic gene cluster for rebeccamycin, an indolocarbazole antibiotic, from Lechevalieria aerocolonigenes ATCC 39243 has 11 ORFs. To clarify their functions, mutants with rebG, rebD, rebC, rebP, rebM, rebR, rebH, rebT, or orfD2 disrupted were constructed, and the gene products were examined. rebP disruptants produced 11,11'-dichlorochromopyrrolic acid, found to be a biosynthetic intermediate by a bioconversion experiment. Other genes encoded N-glycosyltransferase (rebG), monooxygenase (rebC), methyltransferase (rebM), a transcriptional activator (rebR), and halogenase (rebH). rebT disruptants produced rebeccamycin as much as the wild strain, so rebT was probably not involved in rebeccamycin production. Biosynthetic genes of staurosporine, an another indolocarbazole antibiotic, were cloned from Streptomyces sp. TP-A0274. staO, staD, and staP were similar to rebO, rebD, and rebP, respectively, all of which are responsible for indolocarbazole biosynthesis, But a rebC homolog, encoding a putative enzyme oxidizing the C-7 site of pyrrole rings, was not found in the staurosporine biosynthetic gene cluster. These results suggest that indolocarbazole is constructed by oxidative decarboxylation of chromopyrrolic acid (11,11'-dichlorochromopyrrolic acid in rebeccamycin) generated from two molecules of tryptophan by coupling and that the oxidation state at the C-7 position depends on the additional enzyme(s) encoded by the biosynthetic genes.

• 社団法人日本農芸化学会の論文
• 2003-01-23

著者

• Otani H

  Faculty Of Agriculture Tottori University

• Furuki T

  Natural History Museum & Institute

• IGARASHI Yasuhiro

  Biotechnology Research Center, Toyama Prefectural University

• FURUMAI Tamotsu

  Biotechnology Research Center, Toyama Prefectural University

• Taniguchi Shun'ichiro

  Dep. Of Molecular Oncology Inst. On Aging And Adaptation Shinshu Univ. Graduate School Of Medicine

• Taniguchi Shin-ichi

  Biotechnology Research Center Toyama Prefectural University

• ONAKA Hiroyasu

  Biotechnology Research Center, Toyama Prefectural University

• Furumai Tamotsu

  Biotechnology Research Center Toyama Prefectural University

• Tomoyuki Fujita

  Division Of Applied Biological Chemistry Graduate School Of Agriculture And Biological Sciences Osak

• Fujita Tomoyuki

  Dep. Of Sciences Of Functional Foods Graduate School Of Agriculture Shinshu Univ.

• Igarashi Yasuhiro

  Biotechnology Research Center Toyama Pref. Univ.

• Onaka H

  Department Of Engineering Toyama Prefectural University

• Onaka Hiroyasu

  Biotechnology Research Center Toyama Prefectural University

• Taniguchi S

  Biotechnology Research Center Toyama Prefectural University

• Taniguchi Shin-ichi

  Department Of Molecular Oncology Institute On Aging And Adaptation Shinshu University Graduate Schoo

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