Genetic Analysis of Pathogenicity and Host-specific Toxin Production of Alternaria alternata Tomato Pathotype by Protoplast Fusion
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概要
- 論文の詳細を見る
Several pathotypes of Alternaria alternata are known to produce host-specific toxins (HSTs) as agents of pathogenicity or virulence. However, investigations into the genetic controls of HST biosynthesis and pathogenicity of Alternaria pathogens have been limited by the lack of a sexual stage in the life cycle of these pathogens. We report here the development of a protoplast fusion system and its use for genetic analysis of HST production and specific pathogenicity of the tomato pathotype of A. alternata that produces AAL-toxin as a HST. Drug-resistant transformants have been isolated by genetic transformation of nonpathogenic A. alternata (strain O-94) and A. alternata tomato pathotype (strain As-27) with vectors conferring resistance to hygromycin B and geneticin, respectively. Protoplasts of the respective transformants were fused by polyethylene glycol treatment or electrofusion. Fusion products were selected by culturing in the presence of both hygromycin B and geneticin, then confirmed by amplification using a polymerase chain reaction with specific primers to the transforming drug-resistance genes. Stable fusants were purified by successive subcultures on selective medium and single-spore isolation. The resultant stable fusants, probably interstrain hybrids, had the same pathogenicity and toxin production as the wild-type strain As-27. These results suggest that protoplast fusion has potential applications for genetic analysis of A. alternata pathogens.
- 日本植物病理学会の論文
- 2001-02-25
著者
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Otani H
Faculty Of Agriculture Tottori University
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Kodama Motoichiro
Faculty Of Agriculture Tottori University
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OTANI Hiroshi
Faculty of Agriculture, Tottori University
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AKAMATSU Hajime
Faculty of Agriculture, Tottori University
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KOHMOTO Keisuke
Faculty of Agriculture, Tottori University
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Otani Hiroshi
Faculty Of Agriculture Tottori University
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Onaka H
Department Of Engineering Toyama Prefectural University
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Kohmoto K
Faculty Of Agriculture Tottori University
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Kohmoto Keisuke
Faculty Of Agriculture Tottori University
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FUKUMASA-NAKAI Yukitaka
The Tottori Mycological Institute
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Fukumasa‐nakai Y
The Tottori Mycological Institute
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Akamatsu Hajime
Laboratory Of Plant Pathology Faculty Of Agriculture Tottori University
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Akamatsu Hajime
Faculty Of Agriculture Tottori University
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