Cytochrome P450 Homolog Is Responsible for C–N Bond Formation between Aglycone and Deoxysugar in the Staurosporine Biosynthesis of Streptomyces sp. TP-A0274
スポンサーリンク
概要
- 論文の詳細を見る
The staurosporine biosynthetic gene cluster in Streptomyces sp. TP-A0274 consists of 15 sta genes. In the cluster, it was predicted that staN, which shows high similarity to cytochrome P450 is involved in C–N bond formation between the nitrogen at N-12 of aglycone and the carbon at C-5′ of deoxysugar. The staN disruptant produced holyrine A instead of staurosporine. The structure of holyrine A is aglycone linking to 2,3,6-trideoxy-3-aminoaldohexose between N-13 and C-1′ of deoxysugar. Holyrine A was converted to staurosporine by the staD disruptant. These results indicate that StaN, cytochrome P450 is responsible for C–N bond formation. This is the first example of C–N bond formation catalyzed by cytochrome P450. In addition, holyrine A was confirmed to be an intermediate of staurosporine biosynthesis, which suggests that the N- and O-methylation at C-3′ and C-4′ takes place after the formation of the C–N bond between C-5′ and N-12 in the biosynthetic pathway.
- 社団法人 日本農芸化学会の論文
著者
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Yoshida Ryuji
College Of Technology Toyama Prefectural University
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Otani H
Faculty Of Agriculture Tottori University
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IGARASHI Yasuhiro
Biotechnology Research Center, Toyama Prefectural University
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FURUMAI Tamotsu
Biotechnology Research Center, Toyama Prefectural University
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ONAKA Hiroyasu
Biotechnology Research Center, Toyama Prefectural University
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Furumai Tamotsu
Biotechnology Research Center Toyama Prefectural University
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Igarashi Yasuhiro
Biotechnology Research Center Toyama Pref. Univ.
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Onaka H
Department Of Engineering Toyama Prefectural University
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Onaka Hiroyasu
Biotechnology Research Center Toyama Prefectural University
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ASAMIZU Shumpei
Biotechnology Research Center, Toyama Prefectural University
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Asamizu Shumpei
Department Of Biotechnology Faculty Of Engineering Toyama Prefectural University
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