Cloning, Sequencing and Expression of the Cellobiose Phosphorylase Gene of Cellvibrio gilvus
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概要
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The cellobiose phosphorylase gene of Cellvibrio gilvus was cloned and sequenced. The gene had a high GC content of 70.6% and encoded a protein with 822 amino acid residues. It was expressed in Escherichia coli JM 109 to yield the active enzyme. Based on the N-terminal amino acid sequence of the native enzyme, it is found that the enzyme is produced without a signal peptide. Only two previously reported enzymes showed significant amino acid sequence homology ; cellobiose phosphorylase of Clostridium stercorarium(61.4%), and cellodextrin phosphorylase also of C. stercorarium(38.2%).
- 社団法人日本生物工学会の論文
- 1998-05-25
著者
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KANEKO Satoshi
National Food Research Institute
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Aimin Liu
National Food Research Institute Kannondai
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Hayashi Kiyoshi
National Food Research Institute Ministry Of Agriculture Forestry And Fisheries
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Kaneko Satoshi
National Food Res. Inst.
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TOMITA HIDEHIRO
National Food Research Institute, Kannondai
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HEBIAO LI
National Food Research Institute, Kannondai
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MIYAKI HIROSHI
National Food Research Institute, Kannondai
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AOYAGI CHIKA
National Food Research Institute, Kannondai
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Li H
Southern Yangtze Univ. Wuxi Chn
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Aoyagi Chika
National Food Research Institute Kannondai
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Miyaki Hiroshi
National Food Research Institute Kannondai
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Kaneko Satoshi
National Food Research Institute Kannondai
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Hayashi Kiyoshi
National Food Research Institute
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Tomita Hidehiro
National Food Research Institute Kannondai
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Liu Aimin
National Food Research Institute, Kannondai
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Li Heibiao
National Food Research Institute, Kannondai
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