Cloning and Nucleotide Sequencing of the Aminopeptidase Gene from Aeromonas caviae T-64
スポンサーリンク
概要
- 論文の詳細を見る
The aminopeptidase (apAC) gene from Aeromonas caviae T-64 was cloned and sequenced. This gene codes for a polypeptide composed of 393 amino acids with a calculated molecular mass of 42.2kDa. The deduced polypeptide contains a putative signal sequence followed by a large proprotein which is thought to be processed to the mature 29.7kDa protein. The deduced amino acid sequence showed 56.7% identity with that of the Vibrio proteolyticus aminopeptidase, a metalloenzyme with a capacity for binding two Zn^<2+> per molecule. The C-terminal 'helper domain' considered as a requirement for protein excretion, associated with extracellular proteases from the Vibrionaceae family, was not found in apAC. Accordingly, apAC secretion is probably mediated by a different mechanism.
- 公益社団法人日本生物工学会の論文
- 1996-12-25
著者
-
Izawa Noboru
(present Address)milk Production Development Research Institute The National Federation Of Dairy Coo
-
Hayashi Kiyoshi
National Food Research Institute
関連論文
- The Role of Conserved Arginine Residue in Loop 4 of Glycoside Hydrolase Family 10 Xylanases
- Cloning and Expression of Chitin Deacetylase Gene from a Deuteromycete, Colletotrichum lindemuthianum
- PCR Cloning and Expression of the F/10 Family Xylanase Gene from Streptomyces olivaceoviridis E-86
- Substrate Specificity of the α-L-Arabinofuranosidase from Trichoderma reesei
- 647 A putative proline iminopeptidase of Thermotoga maritime with leucine- and lysine-p-nitroanilide hydrolyzing activities
- Syntheses of 4-Methylumbelliferyl-β-D-Xylobioside and 5-Bromo-3-Indolyl-β-D-Xylobioside for Sensitive Detection of Xylanase Activity on Agar Plates
- Purification and Characterization of a Family G/11 β-Xylanase from Streptomyces olivaceoviridis E-86
- Cloning, Sequencing and Expression of the Cellobiose Phosphorylase Gene of Cellvibrio gilvus
- Characterization of a Thermostable Family 10 Endo-Xylanase (XynB) from Thermotoga maritima That Cleaves p-Nitrophenyl-β-D-Xlyloside
- Characterization of Cellobiose Phosphorylase and Cellodextrin Phosphorylase (糖質関連酵素化学シンポジウム)
- Kinetic Studies on the Hydrolysis of N-Acetylated and N-Deacetylated Derivatives of 4-Methylumbelliferyl Chitobioside by the Family 18 Chitinases ChiA and ChiB from Serratia Marcescens
- Purification and Properties of Inulin Fructotransferase (Depolymerizing) from Arthrobacter globiformis C11-1(Microbiology & Fermentation Industry)
- Debittering of Enzymatic Hydrolysates Using an Aminopeptidase from the Edible Basidiomycete Grifola frondosa
- Purification and Characterization of an Aminopeptidase from the Edible Basidiomycete Grifola frondosa
- Importance of Five Amino Acid Residues at C-Tarminal Region for the Folding and Stability of β-Glucosidase of Cellvibrio gilvus
- Cloning and Expression of the N-Acetylmuramidase Gene from Streptomyces rutgersensis H-46
- Bacteriolytic Enzyme Produced by Streptomyces sp. : Control of Microorganisms by Means of Lytic Enzymes (II) For Part I, see ref. 6.
- Taxonomical Studies on a Strain of Streptomyces Producing Glucose Isomerase
- Effects of N-Acetylmuramidase from Streptomyces rutgersensis H-46 as a Food Preservative(Food & Nutrition)
- Cultural Conditions for Lytic Enzyme Production by Streptomyces rutgersensis : Control of Microorganisms by Means of Lytic Enzyme.(IV). For Part III, see ref.11.
- A Single Amino Acid Substitution Enhances the Catalytic Activity of Family 11 Xylanase at Alkaline pH
- Cloning of Inulin Fructotransferase(DFA III-Producing)Gene from Arthrobacter globiformis C11-1
- Cloning and Nucleotide Sequencing of the Aminopeptidase Gene from Aeromonas caviae T-64
- Mutational Analysis of Fungal Family 11 Xylanases on pH Optimum Determination
- Characterization of a Bacterial Laminaribiose Phosphorylase
- Subunit Structure of Glucose Isomerase from Streptomyces griseofuscus, S-41
- Role of Cobalt in Stabilizing the Molecular Structure of Glucose Isomerase from Streptomyces griseofuscus S-41
- Physicochemical Characterization of N-Acetylmuramidase from Streptomyces rutgersensis H-46
- Purification and Characterization of the Lytic Enzyme Produced by Streptomyces rutgersensis H-46
- Properties of N-Acetylmuramidase from Streptomyces rutgersensis H-46