PCR Cloning and Expression of the F/10 Family Xylanase Gene from Streptomyces olivaceoviridis E-86
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概要
- 論文の詳細を見る
Using a simple long-range inverse PCR method, we cloned the GC-rich gene (68%) for an F/10 xylanase from Streptomyces olivaceoviridis E-86. The open reading frame of the cloned gene, fxyn, contained 1431bp and encoded 477 amino acid residues. FXYN resembled a xylanase of the F/10 family and had two functional domains (a catalytic domain and a substrate-binding domain). Unique triple repeat sequence regions (CLD-C) were located in the substrate-binding domain, which was similar to the xylan-binding domains of xylanase A and that of arabinofuranosidase B from S.lividans. FXYN with a tag that consisted to six histidine residues at the carboxy-terminus was expressed at high levels in Escherichia coli and had the same properties as the native xylanase produced by S.olivaceoviridis. Moreover, the xylan-binding domain of FXYN significantly enhanced hydrolysis of insoluble xylan whereas it had minimal effect on the hydrolysis of soluble xylan.
- 社団法人日本生物工学会の論文
- 1998-11-25
著者
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KOBAYASHI Hideyuki
National Astronomical Observatory of Japan
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Kobayashi H
National Food Research Institute
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Kusakabe Isao
Institute of Applied Biochemistry, University of Tsukuba
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YOSHIDA Shigeki
Institute of Applied Biochemistry, University of Tsukuba
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KANEKO Satoshi
Food Biotechnology Division, National Food Research Institute
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Kaneko Satoshi
Food Biotechnology Div. National Food Res. Inst.
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Kuno Atsushi
Research Center For Medical Glycoscience National Institute Of Advanced Industrial Science And Techn
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KANEKO Satoshi
National Food Research Institute
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Kobayashi H
National Food Res. Inst. Ibaraki Jpn
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KUNO Atsushi
Institute of Applied Biochemistry, University of Tsukuba
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Yoshida S
Plant Science Center Riken Institute
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Yoshida S
Cancer Immunotherapy Center Nagoya Kyoritu Hospital
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Yoshida S
Laboratory Of Cancer Cell Biology Research Institute For Disease Mechanism And Control Nagoya Univer
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Kiuchi Hiroshi
Research Development Section Kibun Food Chemifa Co. Ltd.
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Hayashi Kiyoshi
National Food Research Institute Ministry Of Agriculture Forestry And Fisheries
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Hayashi K
National Food Research Institute Ministry Of Agriculture Forestry And Fisheries
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SHIMIZU DAISUKE
Institute of Applied Biochemistry, University of Tsukuba, Tsukuba Science
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KOYAMA YOSHINORI
National Institute of Biocience & Human Technology, Tsukuba Science
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TAIRA KAZUNARI
Institute of Applied Biochemistry, University of Tsukuba, Tsukuba Science
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Kaneko Satoshi
National Food Res. Inst.
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Kaneko S
Food Biotechnology Division National Food Research Institute
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Taira Kazunari
Gene Function Research Center National Institute Of Advanced Industrial Science And Technology (aist
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Taira K
Department Of Biomolecular Engineering Graduate School Of Bioscience And Biotechnology Tokyo Institu
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Taira K
Department Of Chemistry And Biotechnology Graduate School Of Engineering The University Of Tokyo
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Suzuki Y
Department Of Applied Biological Chemistry The University Of Tokyo
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Yoshida Shigeki
Inst. Applied Biochem. Tsukuba Univ.
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Koyama Yoshinori
National Institute Of Biocience & Human Technology Tsukuba Science
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Kaneko Shigenobu
National Institute Of Crop Science (nics)
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Hayashi K
Department Of Biochemical Sciences Kagoshima University
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Kaneko Satoshi
Food Biotechnology Division National Food Research Institute
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Kusakabe I
Univ. Tsukuba Tsukuba Jpn
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Kusakabe Isao
Institute Of Applied Biochemistry University Of Tsukuba
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Taira Kazunari
Institute Of Applied Biochemistry University Of Tsukuba Faculty Of Pharmaceutical Sciences Hokkaido
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Yoshida Shigeki
Institute Of Applied Biochemistry University Of Tsukuba
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Shimizu Daisuke
Institute Of Applied Biochemistry University Of Tsukuba Tsukuba Science:national Institute Of Biosci
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Hayashi Kiyoshi
National Food Research Institute
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