Detection of α-L-Arabinofuranosidase Activity in Isoelectric Focused Gels using 6-Bromo-2-naphthyl-α-L-arabinofuranoside
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概要
- 論文の詳細を見る
For the specific detection of α-L-arabinofuranosidase (α-L-AFase)activity in isoelectric focused gels, 6-bromo-2-naphthyl-α-L-arabino-furanoside (BN-α-L-Araf) was synthesized by the condensation of 2, 3, 5-tri-0-benzoyl-α-L-arabinofuranosyl bromide and 6-bromo. 2-naphthol. α-L-AFase activity had been detected in a gel after isoelectric focusing by using the synthesized BN-α-L-Araf as a substrate, and the detection for the enzyme activity was more sensitive than protein detection with Coomassie Brilliant Blue R-250.
- 社団法人日本農芸化学会の論文
- 1994-03-23
著者
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Kusakabe Isao
Institute of Applied Biochemistry, University of Tsukuba
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YOSHIDA Shigeki
Institute of Applied Biochemistry, University of Tsukuba
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Kawabata Yasuyuki
Institute of Applied Biochemistry, University of Tsukuba
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Kaneko Satoshi
Food Biotechnology Div. National Food Res. Inst.
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Yoshida S
Plant Science Center Riken Institute
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KANEKO Satoshi
Institute of Applied Biochemistry, University of Tsukuba
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Kaneko Satoshi
National Food Res. Inst.
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Kaneko S
Food Biotechnology Division National Food Research Institute
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Nagamoto Yumi
Institute of Applied Biochemistry, University of Tsukuba
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Yoshida Shigeki
Inst. Applied Biochem. Tsukuba Univ.
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Kusakabe I
Univ. Tsukuba Tsukuba Jpn
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Koyama Y
National Inst. Biosci. & Human Technol. Tsukuba Science City Jpn
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Nagamoto Yumi
Institute Of Applied Biochemistry University Of Tsukuba
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Kusakabe Isao
Institute Of Applied Biochemistry University Of Tsukuba
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Kawabata Y
Univ. Tsukuba Ibaraki Jpn
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Kawabata Yasuyuki
Institute Of Applied Biochemistry University Of Tsukuba
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Kaneko Satoshi
Institute Of Applied Biochemistry University Of Tsukuba
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Yoshida Shigeki
Institute Of Applied Biochemistry University Of Tsukuba
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