Improvement of the Transformation Efficiency of Flammulina velutipes Fv-1 Using the Glyceraldehyde-3-phosphate Dehydrogenase Gene Promoter
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概要
- 論文の詳細を見る
To improve the expression level of heterologous genes in Flammulina velutipes Fv-1, we constructed new vectors having glyceraldehydes-3-phosphate dehydrogenase (gpd) gene promoter to control the expression of target genes. When the hygromycin B phosphotransferase (hph) gene from Escherichia coli was controlled by the gpd promoter, transformation efficiency was 3-fold higher than the case of that controlled by the tryptophan synthetase gene (trp1) promoter.
- 社団法人 日本農芸化学会の論文
- 2010-12-23
著者
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TAKABATAKE Koji
Toyama Prefectural Agricultural, Forestry and Fisheries Research Center, Forest Research Institute
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KANEKO Satoshi
Food Biotechnology Division, National Food Research Institute
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Kaneko Satoshi
Food Biotechnology Div. National Food Res. Inst.
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KANEKO Satoshi
National Food Research Institute
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Kaneko Satoshi
National Food Res. Inst.
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Takabatake Koji
Toyama Prefectural Agricultural Forestry And Fisheries Research Center Forest Research Institute
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Kaneko Shigenobu
National Institute Of Crop Science (nics)
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Kaneko Satoshi
Food Biotechnology Division National Food Research Institute
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MAEHARA Tomoko
Food Biotechnology Division, National Food Research Institute
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TOMITA Shizuko
Food Biotechnology Division, National Food Research Institute
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TAKABATAKE Koji
Forest Institute, Toyama Prefectural Agricultural, Forestry, and Fisheries Research Center
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Maehara Tomoko
Food Biotechnology Division National Food Research Institute
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Tomita Shizuko
Food Biotechnology Division National Food Research Institute
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