Catalytic Activity of Tripeptidase from Lactococcus lactis to Which Amino Acid Substitution Was Introduced According to Natural Mutation
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概要
- 論文の詳細を見る
Four mutations observed between tripeptidases from Lactococcus lactis subsp. lactis and subsp. cremoris were introduced one by one to the corresponding points in wild-type tripeptidase from L. lactis subsp. lactis. The kcat values of four resultant mutants were analyzed and discussed in stereographical terms. Change in catalytic activity appeared to be related to the sequential and steric location of mutation point within the enzyme protein, even though no drastic change was observed with one point mutation.
- 社団法人 日本農芸化学会の論文
- 2004-05-23
著者
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Kaneko Satoshi
National Food Res. Inst.
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KASUMI Takafumi
National Food Research Institute
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Kasumi T
National Food Res. Inst. Ibaraki Jpn
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MORI Sumiko
National Food Research Institute
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Mori S
Faculty Of Agriculture Ehime University
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